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Gene and DNA Sequence
Record information and status
Record ID
14974
Status
Published
Date of creation
2006-06-09 16:17 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2014-02-26 20:29 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-02-26 20:29 UTC (dina.abdelhakim@cbd.int)

General information
Name of the gene or DNA sequence
Barstar
Abbreviation
CS-Barstar-BACAS
Category of DNA sequence
  • Protein coding sequence
Is the gene / DNA sequence is a synthetic molecule?
No
Donor organism
Donor organism
Bacillus amyloliquefaciens - BACAS
Characteristics of the protein coding sequence
Name of the protein expressed by the coding sequence
barstar ribonuclease inhibitor
Biological function
The barstar gene from Bacillus amyloliquefaciens encodes a specific ribonuclease inhibitor (barstar enzyme) expressed only in the tapetum cells of the pollen sac during anther development.

The barstar enzyme specifically inhibits barnase RNAse. Together, the barnase RNAse and the barstar form a very stable one-to-one complex, in which the RNAse is inactivated. As a result, when pollen from the restorer line is crossed to the male sterile line, the resultant progeny express the RNAse inhibitor in the tapetum cells of the anthers allowing hybrid plants to develop normal anthers and restore fertility.

Also, the barstar gene may be used to prevent the barnase RNAse from disrupting the development of bacteria in which the introduced DNA was prepared during the transformation process.
Related trait(s) or use(s) in biotechnology
Additional Information
Other relevant website address or attached documents

Records referencing this document (101)
IDDescription
101record(s) found
Country's Decision or any other Communication53 records
Modified Organism14 records
Organization3 records
Risk Assessment31 records