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Modified Organism
BPS-25271-9 - Amflora™Potato
Record information and status
Record ID
15100
Status
Published
Date of creation
2006-06-19 09:49 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-05-13 19:17 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-05-13 19:17 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Amflora™Potato
Transformation event
EH92-527-1
Unique identifier
BPS-25271-9
Description
Amflora™Potato was modified to produce less amylose starch and thus a higher content of amylopectin. This was done through anti-sense suppression of the granule bound starch synthase (GBSS) protein.

GBSS enzyme is one of the key enzymes in the biosynthesis of starch and catalyses the formation of amylose. When the gene is inactivated through antisense technology, the starch produced has little or no amylose and consists of branched amylopectin, which modifies the physical properties of the starch and is advantageous for the starch processing industry.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Characteristics of the transformation process
Vector
pHoxwG
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Granule bound starch synthase gene promoter
0.99 Kb
 
 
Granule-bound starch synthase gene
1.94 Kb
 
 
Nopaline Synthase Gene Terminator
0.28 Kb
 
 
Nopaline Synthase Gene Promoter
0.37 Kb
 
 
Neomycin Phosphotransferase II
0.98 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The Granule-bound starch synthase gene was inserted in an anti-sense orientation, relative to the promoter therefore resulting in the suppression of endogenous GBSS.

Southern blot analysis indicated that a single copy of the insert was integrated into the host genome with no integration of fragments of the vector backbone.
LMO characteristics
Modified traits
  • Reduced amylose in starch content
Common use(s)
  • Industrial
Additional Information
Other relevant website address or attached documents

Records referencing this document (21)
IDDescription
21record(s) found
Country's Decision or any other Communication4 records
Modified Organism2 records
Organization9 records
Risk Assessment6 records