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Gene and DNA Sequence
Record information and status
Record ID
15164
Status
Published
Date of creation
2006-06-20 09:57 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-09-24 19:10 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-09-24 19:10 UTC (dina.abdelhakim@cbd.int)

General information
Name of the gene or DNA sequence
Acetohydroxy acid synthase gene (Chimeric)
Alternate Genetic Element Name (synonyms)
Acetolactate Synthase
Abbreviation
CS-S4-HrA
Category of DNA sequence
  • Protein coding sequence
Is the gene / DNA sequence is a synthetic molecule?
No
Donor organism
Donor organism
Nicotiana tabacum - Tobacco, TOBAC
Donor organism point of collection
Nicotiana tabacum cv. Xanthi
Characteristics of the protein coding sequence
Name of the protein expressed by the coding sequence
Chimeric Acetohydroxy acid synthase
Biological function
Acetolactate synthase (ALS) catalyzes the first common step in the biosynthesis of the essential amino acids isoleucine, leucine, and valine.

Native ALS is normally inhibited by sulfonylurea herbicides. However S4-HrA is a chimeric gene derived from two different tobacco ALS genes that both encoded herbicide sensitive versions of ALS.

Two resistance mutations were introduced into one of the ALS genes (Pro191Ala and Trp568Leu) via site directed mutagenesis. The DNA fragment containing the resistance mutations was then moved into the second ALS gene through a restriction enzyme fragment.
Related trait(s) or use(s) in biotechnology

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Modified Organism1 record