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Modified Organism
DAS-59122-7 x MON-ØØ6Ø3-6 - Herculex™ RW Rootworm Protection Roundup Ready™ 2 maize
Record information and status
Record ID
15178
Status
Published
Date of creation
2006-06-20 19:59 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-07-24 14:21 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-07-24 14:21 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herculex™ RW Rootworm Protection Roundup Ready™ 2 maize
Transformation event
59122 x NK603
Unique identifier
DAS-59122-7 x MON-ØØ6Ø3-6
Developer(s)
Pioneer Hi-Bred International Inc.
Url:Pioneer HiBred International Homepage
Description
The stacked maize line 59122 x NK603 was produced by cross breeding between maize LMOs 59122 and NK603 to resist the Coleopteran pests (western corn rootworm, northern corn rootworm and Mexican corn rootworm) and for tolerance to herbicides containing glufosinate and glyphosate.

59122 x NK603 inherited the cry34Ab1, cry35Ab1 and pat genes from the 59122 parent, and two copies of the epsps gene from NK603
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
DAS-59122-7 - Herculex™ RW Rootworm Protection maize
Resistance to diseases and pests - Insects - Coleoptera (beetles) Resistance to herbicides - Glufosinate
Show detection method(s)
MON-ØØ6Ø3-6 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
PV-ZMGT32 and PHP17662
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Ubiquitin gene promoter
0.00 Kb
 
 
Cry34Ab1
0.00 Kb
 
 
Proteinase inhibitor II gene terminator
0.00 Kb
 
 
Peroxidase gene promoter
0.00 Kb
 
 
Cry35Ab1
0.00 Kb
 
 
Proteinase inhibitor II gene terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
 
Rice actin 1 gene promoter
0.80 Kb
 
 
Rice actin 1, intron
0.60 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
 
CaMV Enhanced 35S promoter
0.60 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from 59122 vector PHP17662:
The cry34Ab1 and cry35Ab1 genes, isolated from the common soil bacterium Bacillus thuringiensis (Bt) strain PS149B1, produce the insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1.

The pat gene was isolated from the soil bacterium Streptomyces viridochromogenes and confers tolerance to herbicides containing glufosinate ammonium.

DNA insert from NK603 vector PV-ZMGT32:
59122 x NK603 also contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the plant to survive the otherwise lethal application of glyphosate. The glyphosate-tolerant EPSPS gene was isolated from the CP4 strain of the common soil bacterium Agrobacterium tumefaciens.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Biofuel
Additional Information

Records referencing this document (19)
IDDescription
19record(s) found
Country's Decision or any other Communication6 records
Information Resource1 record
Organization6 records
Risk Assessment6 records