Herculex™ RW Rootworm Protection Roundup Ready™ 2 maize

The stacked maize line 59122 x NK603 was produced by cross breeding between maize LMOs 59122 and NK603 to resist the Coleopteran pests (western corn rootworm, northern corn rootworm and Mexican corn rootworm) and for tolerance to herbicides containing glufosinate and glyphosate.

59122 x NK603 inherited the cry34Ab1, cry35Ab1 and pat genes from the 59122 parent, and two copies of the epsps gene from NK603

PV-ZMGT32 and PHP17662

DNA insert from 59122 vector PHP17662:
The cry34Ab1 and cry35Ab1 genes, isolated from the common soil bacterium Bacillus thuringiensis (Bt) strain PS149B1, produce the insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1.

The pat gene was isolated from the soil bacterium Streptomyces viridochromogenes and confers tolerance to herbicides containing glufosinate ammonium.

DNA insert from NK603 vector PV-ZMGT32:
59122 x NK603 also contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the plant to survive the otherwise lethal application of glyphosate. The glyphosate-tolerant EPSPS gene was isolated from the CP4 strain of the common soil bacterium Agrobacterium tumefaciens.

For additional information on this LMO, please refer to the records of the parental LMOs.