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Modified Organism
MON-88913-8 x MON-15985-7 - Roundup Ready™ Flex™ Bollgard II™ cotton
Record information and status
Record ID
15215
Status
Published
Date of creation
2006-06-23 09:37 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2014-02-12 21:40 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-02-12 21:40 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Roundup Ready™ Flex™ Bollgard II™ cotton
Transformation event
88913 x 15985 (MON88913 x MON15985)
Unique identifier
MON-88913-8 x MON-15985-7
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
The stacked cotton line MON88913 x MON15985 expresses several novel proteins: the delta-endotoxins the Cry1Ac and Cry2Ab which confer resistance to lepidopteran pests, and EPSPS (5-enolpyruvylshikimaete-3-phosphate synthase) that confers tolerance to the herbicide glyphosate.

The cry1Ac gene and the cry2Ab gene from Bacillus thuringiensis subsp. kurstaki conferring resistance to lepidopteran pests, and the epsps gene encoding 5-enolpyruvylshikimaete-3-phosphate synthase that confers tolerance to the herbicide glyphosate. gene from Agrobacterium tumefaciens strain CP4.

The cry1Ac and cry2Ab genes, isolated from the common soil bacterium Bacillus thuringiensis (Bt) strain subsp. kurstaki, produce the insect control proteins (delta-endotoxins) Cry1Ac and Cry2Ab. Cry proteins, of which these are only two among many, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis. There are no binding sites for the delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.

The line also contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the plant to survive the otherwise lethal application of glyphosate. The glyphosate-tolerant EPSPS gene was isolated from the CP4 strain of the common soil bacterium Agrobacterium tumefaciens.

Glyphosate specifically binds to and inactivates the enzyme EPSPS, which is part of an important plant biochemical pathway called the shikimate pathway. The shikimate pathway is involved in the biosynthesis of the aromatic amino acids tyrosine, phenylalanine and tryptophan, as well as other aromatic compounds. When conventional plants are treated with glyphosate they cannot produce the aromatic amino acids essential to their survival. The modified maize line permits farmers to use glyphosate-containing herbicides for weed control in the cultivation of maize. The EPSPS enzyme is present in all plants, bacteria and fungi, but not in animals, which do not synthesize their own aromatic amino acids. Thus, EPSPS is normally present in food derived from plant and microbial sources.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
MON-88913-8 - Roundup Ready™ Flex™ cotton
Resistance to herbicides - Glyphosate
Show detection method(s)
MON-15985-7 - Bollgard II™ cotton
Resistance to antibiotics - Kanamycin, Streptomycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
Show detection method(s)
Related LMOs
MON-ØØ531-6 - Bollgard™ cotton
Resistance to antibiotics - Kanamycin, Streptomycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
Characteristics of the transformation process
Vector
PV-GHGT35, PV-GHBK04 and PV-GHBK11
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
FMV 35S Enhancer
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Elongation factor EF-1alpha promoter
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Elongation factor EF-1alpha Leader
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Elongation factor EF-1alpha Intron 1
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Chloroplast transit peptide 2
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5-enolpyruvylshikimate-3-phosphate synthase gene
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rbcS-E9 gene terminator
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CaMV 35S Enhancer
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Actin 8 Promoter
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Actin 8 Leader sequence
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Actin 8 Intron 1
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Chloroplast transit peptide 2
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5-enolpyruvylshikimate-3-phosphate synthase gene
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rbcS-E9 gene terminator
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CaMV Enhanced 35S promoter
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HSP 70 5' untranslated leader sequence
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Chloroplast transit peptide 2
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Cry2Ab2
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Nopaline Synthase Gene Terminator
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CaMV Enhanced 35S promoter
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Cry1Ac
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α' subunit of β-conglycinin gene terminator
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CaMV Enhanced 35S promoter
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Neomycin Phosphotransferase II
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Nopaline Synthase Gene Terminator
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CaMV Enhanced 35S promoter
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Beta-Glucuronidase coding sequence
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Nopaline Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
A stacked insect-resistant and herbicide-tolerant cotton derived by crossing MON-88913-8 with MON-15985-7, including the cry1Ac gene and the cry2Ab gene from Bacillus thuringiensis subsp. kurstaki conferring resistance to lepidopteran pests, and the epsps gene encoding 5-enolpyruvylshikimaete-3-phosphate synthase that confers tolerance to the herbicide glyphosate. The neomycin phosphotransferase II (npt II) gene confers resistance to the antibiotic kanamycin and was used as a selectable marker.

DNA insert from MON 88913 vector PV-GHGT35
Roundup Ready® Flex cotton (MON 88913) was developed to allow the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option in cotton production. This genetically engineered cotton contains a novel form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows MON 88913 to survive otherwise lethal applications of glyphosate. The EPSPS gene introduced into MON 88913 was isolated from a strain of the common soil bacterium Agrobacterium tumefaciens strain CP4; the EPSPS enzyme expressed by this gene is tolerant to glyphosate. MON 88913 cotton contains two copies of the EPSPS gene to confer tolerance to glyphosate later in the growing season, specifically after the fifth true leaf stage. 

DNA insert from MON15985 vector PV-GHBK11 and PV-GHBK04
Event 15985 (tradename Bollgard II®) was derived from the retransformation of transgenic cotton line MON 531. As a result of these two transformation events, MON15985 contains the cry1Ac gene and the cry2Ab conferring resistance to lepidopteran pests as well as copies of the nptII and uidA and aadA genes.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Fiber / Textile
Additional Information
Other relevant website address or attached documents

Records referencing this document (182)
IDDescription
182record(s) found
Country's Decision or any other Communication87 records
Information Resource1 record
Modified Organism1 record
Organization2 records
Risk Assessment91 records