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Modified Organism
SEM-ØZW2Ø-7 - Virus-resistant squash
Record information and status
Record ID
15218
Status
Published
Date of creation
2006-06-23 10:04 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2012-12-06 22:00 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-12-06 22:00 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Virus-resistant squash
Transformation event
ZW20
Unique identifier
SEM-ØZW2Ø-7
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Seminis Vegetable Seeds, Inc.
Url:Seminis Homepage
Description
Squash resistant to infection by Zucchini yellow mosaic potyvirus (ZYMV) and Watermelon mosaic potyvirus 2 (WMV-2) through incorporation of virus-derived sequences that encode the coat proteins (CPs) from both of these viruses.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Cucurbita pepo - Pumpkin, Squashes
Related LMOs
SEM-ØCZW3-2 - Virus-resistant squash
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Viruses - Mosaic virus - Cucumber mosaic virus (CMV), Watermelon mosaic virus-2 (WMV2), Zucchini yellow mosaic virus (ZYMV)
Show detection method(s)
Characteristics of the transformation process
Vector
ZYMV72/WMBN22
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
WMV-2 coat protein
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CaMV 35S terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
ZYMV coat protein
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The WMV2 CP gene was fused to the 5' intergenic region and the first 48 nucleotides (N-terminus) of the CMV coat protein gene to enhance translation of the transgene mRNA.

The ZYMV CP coding sequence was fused to the CMV 5' untranslated region to enhance translation.

While the gene encoding neomycin phosphotransferase (NPTII) was incorporated into the Ti plasmid vector used for transformation, only plants lacking this gene were selected for commercialization. The gene encoding NPTII is, therefore, not present in the ZW-20 genome. There was no incorporation of plasmid DNA sequences outside of the T-DNA region as verified by Southern blot analysis.
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Additional Information
The ZW20 squash line was developed using recombinant DNA techniques to resist infection by ZYMV and WMV2 by inserting virus-derived sequences that encode the coat proteins (CPs) from each of these viruses. The introduced viral sequences do not result in the formation of any infectious particles, nor does their expression result in any disease pathology.

This transgenic squash exhibits "pathogen-derived resistance" to infection and subsequent disease caused by ZYMV and WMV2 through a process that is related to viral cross-protection. Although the exact mechanism by which the viral protection occurs is unknown, most evidence suggests that expression of viral CP by a plant interferes with one of the first steps in viral replication, uncoating (removal of CP) from the incoming virus. Other modes of action of cross-protection have also been suggested.
Other relevant website address or attached documents

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Modified Organism1 record