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Modified Organism
ACS-ZMØØ3-2 x MON-ØØ81Ø-6 - Liberty Link™ Yieldgard™ maize
Record information and status
Record ID
15373
Status
Published
Date of creation
2006-07-12 15:52 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-07-24 14:40 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-07-24 14:40 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Liberty Link™ Yieldgard™ maize
Transformation event
T25 x MON810
Unique identifier
ACS-ZMØØ3-2 x MON-ØØ81Ø-6
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
The stacked maize line ACS-ZMØØ3-2 x MON-ØØ81Ø-6 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of PAT and Cry1Ab genes. The expression of these genes are expected to confer resistance to Lepidoptera, and tolerant to glufosinate herbicide.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
ACS-ZMØØ3-2 - Liberty Link™ maize
Resistance to antibiotics - Ampicillin Resistance to herbicides - Glufosinate
Show detection method(s)
MON-ØØ81Ø-6 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
Characteristics of the transformation process
Vector
pDH51, PV-ZMBK07 and PV-ZMGT10
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Beta-lactamase gene
0.86 Kb
 
 
pUC origin of replication
2.63 Kb
 
 
CaMV 35S promoter
0.52 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.53 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
 
CaMV Enhanced 35S promoter
0.61 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Cry1Ab
3.46 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from ACS-ZMØØ3-2 vector pDH51
Glufosinate tolerance in T25 maize due to the enzyme phosphinothricin-N-acetyltransferase (PAT). The PAT enzyme catalyzes the acetylation of phosphinothricin, detoxifying it into an inactive compound. Molecular analyses shows that it has a truncated copy of the bla gene (25% of the 5' end of the bla gene is missing in T25) and an intact Ori-pUC.

DNA insert from MON-ØØ81Ø-6 vector PV-ZMBK07 and PV-ZMGT10
MON810 contains a truncated portion of a synthetic form of the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki. This confers resistance to Lepidoptera pests. Two constructs PV-ZMBK07 and PV-ZMGT10 were used for transformation, but molecular analyses showed no elements from the PV-ZMGT10 construct were integrated and only the elements from construct PV-ZMBK07 have been integrated into its genome. The terminator of the nopaline synthase (nos) gene was lost due to a truncation at the 3' end of the gene cassette during genome integration and is, therefore, not present in MON810.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (9)
IDDescription
9record(s) found
Country's Decision or any other Communication1 record
Organization7 records
Risk Assessment1 record