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Modified Organism
Chicory RM3-6 modified for tolerance to herbicides
Record information and status
Record ID
15404
Status
Published
Date of creation
2006-07-13 15:04 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2012-09-24 18:42 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-09-24 18:42 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Chicory RM3-6 modified for tolerance to herbicides
Transformation event
RM3-6
Developer(s)
Description
Chicory male-sterility system displaying glufosinate herbicide tolerance. Contains the barnase gene from Bacillus amyloliquefaciens and the bar gene encoding phosphinothricin N-acetyltransferase from Streptomyces hygroscopicus to confer tolerance to the herbicide phosphinothricin (Glufosinate ammonium).
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Cichorium intybus - Chicory
Related LMOs
Chicory RM3-4 modified for tolerance to herbicides
Changes in physiology and/or production - Reproduction - Male sterility Resistance to antibiotics - Kanamycin Resistance to herbicides - Glufosinate
Chicory RM3-3 modified for tolerance to herbicides
Changes in physiology and/or production - Reproduction - Male sterility Resistance to antibiotics - Kanamycin Resistance to herbicides - Glufosinate
Characteristics of the transformation process
Vector
pTTM8RE derived from pGV2260
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
rbcS Promoter
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rbcS Transit Peptide
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Phosphinothricin N-acetyltransferase gene
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Transcript 7 gene 3' untranslated region
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pTA29 pollen specific promoter
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Barnase
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Nopaline Synthase Gene Terminator
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Nopaline Synthase Gene Promoter
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Neomycin Phosphotransferase II
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Octopine Synthase Gene Terminator
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Further details
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Additional Information
Radicchio Rosso lines RM3-3, RM3-4, and RM3-6 (male sterile chicory plants) contain a ribonuclease encoded by barnase gene. The ribonuclease, which is expressed only in the tapetum cells of the pollen sac during anther development, blocks pollen development, thus producing a male sterile plant. To direct the expression of barnase to pollen cells, a pollen specific promoter, pTA 29 was used to direct the synthesis of ribonuclease enzyme.

Expression of barnase in pollen cells apparently results in degradation of host RNAs and blocking development of mature viable pollen. The barnase gene encodes for an extracellular ribonuclease protein in Bacillus amyloliquefaciens, a common soil microorganism. B. amyloliquefaciens also contains an intracellular protein called barstar which specifically inhibits barnase by combining with it in a one-to-one complex. Thus, barstar is produced intracellularly by the same organism that secretes barnase.

The second gene (bar) inserted into the Radicchio Rosso lines RM3-3, RM3-4, and RM3-6 genome encodes for phosphinothricin acetyltransferase (PAT) enzyme which inactivates phosphinothricin, the active component in the herbicide glufosinate. The bar gene is produced in the lines under the direction of Arabidospsis thaliana PssuAra promoter and nopaline synthase termination/polyadenylation sequences. Although these lines are tolerant to the herbicide, hybrid progeny (the seeds that would be available to the farmers) developed from these lines may or may not be tolerant to the herbicide.
Other relevant website address or attached documents

Records referencing this document (3)
IDDescription
3record(s) found
Country's Decision or any other Communication1 record
Modified Organism2 records