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Modified Organism
Tobacco modified for reduced nicotine levels
Record information and status
Record ID
15417
Status
Published
Date of creation
2006-07-13 17:00 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2012-12-04 21:59 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-12-04 21:59 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Tobacco modified for reduced nicotine levels
Transformation event
Vector 21-41
Developer(s)
Vector Tobacco Ltd
Url:Vector Tobacco Homepage
Description
Tobacco which expresses a low level of nicotine through incorporation of an antisense version of the Quinolinic Acid Phosphoribosyltransferase gene (NtQPT1) which codes for for quinolinic acid phosphoribosyltransferase (QPTase). The nptII gene confers resistance to the antibiotic kanamycin and was used as a marker gene.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Nicotiana tabacum - Tobacco, TOBAC
Point of collection or acquisition of the recipient organism
Tobacco variety Burley 21-LA
Characteristics of the transformation process
Vector
pYTY32 derived from pBin19
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ti plasmid right border repeat
0.16 Kb
 
 
Nopaline Synthase Gene Promoter
0.31 Kb
 
 
Neomycin Phosphotransferase II
0.98 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
Quinolinic Acid Phosphoribosyltransferase gene Promoter
2.01 Kb
 
 
Quinolinic Acid Phosphoribosyltransferase gene
1.35 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
Ti plasmid left border repeat
0.15 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Vector 21-41 tobacco has been genetically engineered to express a quinolinic acid phosphoribosyltransferase (QPTase) in the reverse, or antisense position, which disrupts the normal expression of QPTase, a key enzyme in the biosynthetic pathway leading to the production of nicotine and related alkaloids. The effect of this genetic change is to reduce the nicotine levels of nicotine, nor-nicotine, and total alkaloids in the leaves of Vector 21-41 tobacco.

The tobacco line also contains the nptII marker gene derived from the bacterium Escherichia coli. The nptII gene encodes the enzyme neomycin phosphotransferase type II (NPTII) and is used as a selectable marker in the initial laboratory stages of plant cell selection. Expression of the added genes is controlled in part by gene sequences from the plant pathogen Agrobacterium tumefaciens. The A. tumefaciens method was used to transfer the added genes into the parental recipient  tobacco variety.
LMO characteristics
Modified traits
  • Reduced nicotine content
Additional Information
Other relevant website address or attached documents

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