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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Tomato modified for delayed ripening
EN
35-1-N
No
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Organization:Agritope, Inc ()Phone:Fax:Email:Website: http://www.agritope.com/,
Tomato with delayed ripening due to expression of the SAMase from the E. coli bacteriophage T3.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12079-5 Organism Solanum lycopersicum (Tomato, SOLLC)Crops
EN
pAG-5420
EN
- Agrobacterium-mediated DNA transfer
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2.300 kb
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0.510 kb
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0.270 kb
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0.300 kb
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1.020 kb
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1.100 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-15017-5 S-adenosylmethionine hydrolase gene | Bacteriophage T3 (Phage T3, T3)Protein coding sequence | Changes in physiology and/or production (Ripening)
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-104862-2 E8 gene promoter | Solanum lycopersicum (Tomato, SOLLC)Promoter
The sam-k gene was modified to alter the native initiation site to contain a consensus eukaryotic translation initiation site - Kozak sequence.
Southern blot ananlysis indicated that the T-DNA integrated at a single locus within the host genome. Within this site is one complet and on e partial copy of the sam-k coding sequence. Further more fragments of the oriT and TrfA genetic elements from the vector backbone were detected in the host genome.
EN
Southern blot ananlysis indicated that the T-DNA integrated at a single locus within the host genome. Within this site is one complet and on e partial copy of the sam-k coding sequence. Further more fragments of the oriT and TrfA genetic elements from the vector backbone were detected in the host genome.
EN
- Food
EN
The large red cherry tomato Lycopersicon esculentum mill var. cerasiforme has a modified ripening phenotype resulting in the production of a more physiologically mature fruit that is able to withstand the rigors of the current production and distribution system. This was accomplished by inserting the sam-k gene encoding SAMase from the E. coli bacteriophage T3 (under a promoter that directs fruit-specific expression) into the tomato genome. SAMase degrades S-adenosylmethionine (SAM), which is the penultimate precursor in the ethylene biosynthetic pathway.
The nptII enzyme was used as a selectable marker in the production of the transgenic tomatoes.
EN
The nptII enzyme was used as a selectable marker in the production of the transgenic tomatoes.
- 35-1-N - APHIS [ English ]
- 35-1-N - FDA [ English ]
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