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Modified Organism
Tomato modified for delayed ripening
Record information and status
Record ID
15419
Status
Published
Date of creation
2006-07-13 17:28 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2014-04-08 21:30 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-04-08 21:30 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Tomato modified for delayed ripening
Transformation event
35-1-N
Developer(s)
Agritope, Inc
Url:Agritope Homepage
Description
Tomato with delayed ripening due to expression of the SAMase from the E. coli bacteriophage T3.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum lycopersicum - Tomato, SOLLC
Characteristics of the transformation process
Vector
pAG-5420
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
E8 gene promoter
2.30 Kb
 
 
S-adenosylmethionine hydrolase gene
0.51 Kb
 
 
Nopaline Synthase Gene Terminator
0.27 Kb
 
 
Nopaline Synthase Gene Promoter
0.30 Kb
 
 
Neomycin Phosphotransferase II
1.02 Kb
 
 
Nopaline Synthase Gene Terminator
1.10 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The sam-k gene was modified to alter the native initiation site to contain a consensus eukaryotic translation initiation site - Kozak sequence.

Southern blot ananlysis indicated that the T-DNA integrated at a single locus within the host genome. Within this site is one complet and on e partial copy of the sam-k coding sequence. Further more fragments of the oriT and TrfA genetic elements from the vector backbone were detected in the host genome.
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Additional Information
The large red cherry tomato Lycopersicon esculentum mill var. cerasiforme has a modified ripening phenotype resulting in the production of a more physiologically mature fruit that is able to withstand the rigors of the current production and distribution system. This was accomplished by inserting the sam-k gene encoding SAMase from the E. coli bacteriophage T3 (under a promoter that directs fruit-specific expression) into the tomato genome. SAMase degrades S-adenosylmethionine (SAM), which is the penultimate precursor in the ethylene biosynthetic pathway.

The nptII enzyme was used as a selectable marker in the production of the transgenic tomatoes.
Other relevant website address or attached documents

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