Liberty Link™ Bollgard II™ cotton

A stacked insect-resistant and herbicide-tolerant cotton derived from conventional cross-breeding of ACS-GHØØ1-3 with MON-15985-7. Tolerance to the herbicide glufosinate ammonium was produced by inserting a modified phosphinothricin acetyltransferase (PAT) encoding gene (bar) from Streptomyces hygroscopicus, and resistance to lepidoptera insects is conferred from the cry1Ac gene and the cry2Ab gene from Bacillus thuringiensis.

pGSV71, PV-GHBK11 and PV-GHBK04

DNA insert from MON-15985-7 vectors PV-GHBK11 and PV-GHBK04

Event 15985 (tradename Bollgard II®) was derived from the retransformation of transgenic cotton line MON 531. As a result of these two transformation events, MON15985 contains the cry1Ac gene and the cry2Ab conferring resistance to lepidopteran pests as well as copies of the nptII and uidA and aadA genes.


DNA insert from LLCotton25 vector pGSV71

The T-DNA cassette from teh LLCotton25 line contined the coding sequence for the bar gene which confers tolerance to the glufosinate herbicide.

For additional information on this LMO, please refer to the records of the parental LMO.

The stacked cotton line ACS-GHØØ1-3 x MON-15985-7 expresses three novel proteins: the enzyme phosphinothricin acetyl-transferase (PAT) and the delta-endotoxins Cry1Ac and Cry2Ab, both of which confer resistance to the lepidopteran pests of cotton, such as the cotton bollworm, pink bollworm and tobacco budworm.

The insecticidal proteins Cry1Ac and Cry2Ab are produced by the cry1Ac and cry2Ab genes respectively, both of which were originally isolated from the soil bacterium Bacillus thuringiensis and are derived from MON-15985-7. The bar gene, which codes for the production of PAT, is derived from ACS-GHØØ1-3. This enzyme renders glufosinate ammonium inactive in the plant. The expression of the bar gene allows the plant to survive the otherwise lethal application of glufosinate ammonium. The bar gene was isolated from Streptomyces hygroscopius, a gram-positive soil bacterium. The novel traits of each parental line have been combined, through traditional plant breeding, to produce this new cotton line.

For a full description of each parental line please refer to the individual descriptions for the parent organisms.

This plant is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment.