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Modified Organism
ACS-GHØØ1-3 x MON-15985-7 - Liberty Link™ Bollgard II™ cotton
Record information and status
Record ID
30886
Status
Published
Date of creation
2007-01-30 07:06 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2014-02-12 19:16 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-02-12 19:16 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Liberty Link™ Bollgard II™ cotton
Transformation event
LLCotton25 x MON15985
Unique identifier
ACS-GHØØ1-3 x MON-15985-7
Developer(s)
Description
A stacked insect-resistant and herbicide-tolerant cotton derived from conventional cross-breeding of ACS-GHØØ1-3 with MON-15985-7. Tolerance to the herbicide glufosinate ammonium was produced by inserting a modified phosphinothricin acetyltransferase (PAT) encoding gene (bar) from Streptomyces hygroscopicus, and resistance to lepidoptera insects is conferred from the cry1Ac gene and the cry2Ab gene from Bacillus thuringiensis.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
ACS-GHØØ1-3 - Liberty Link™ cotton
Resistance to herbicides - Glufosinate
Show detection method(s)
MON-15985-7 - Bollgard II™ cotton
Resistance to antibiotics - Kanamycin, Streptomycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
Show detection method(s)
Characteristics of the transformation process
Vector
pGSV71, PV-GHBK11 and PV-GHBK04
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
HSP 70 5' untranslated leader sequence
0.00 Kb
 
 
Chloroplast transit peptide 2
0.00 Kb
 
 
Cry2Ab2
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
Cry1Ac
0.00 Kb
 
 
α' subunit of β-conglycinin gene terminator
0.00 Kb
 
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
Beta-Glucuronidase coding sequence
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
CaMV 35S promoter
1.38 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from MON-15985-7 vectors PV-GHBK11 and PV-GHBK04

Event 15985 (tradename Bollgard II®) was derived from the retransformation of transgenic cotton line MON 531. As a result of these two transformation events, MON15985 contains the cry1Ac gene and the cry2Ab conferring resistance to lepidopteran pests as well as copies of the nptII and uidA and aadA genes.


DNA insert from LLCotton25 vector pGSV71

The T-DNA cassette from teh LLCotton25 line contined the coding sequence for the bar gene which confers tolerance to the glufosinate herbicide.

For additional information on this LMO, please refer to the records of the parental LMO.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Fiber / Textile
Additional Information
Additional Information
The stacked cotton line ACS-GHØØ1-3 x MON-15985-7 expresses three novel proteins: the enzyme phosphinothricin acetyl-transferase (PAT) and the delta-endotoxins Cry1Ac and Cry2Ab, both of which confer resistance to the lepidopteran pests of cotton, such as the cotton bollworm, pink bollworm and tobacco budworm.

The insecticidal proteins Cry1Ac and Cry2Ab are produced by the cry1Ac and cry2Ab genes respectively, both of which were originally isolated from the soil bacterium Bacillus thuringiensis and are derived from MON-15985-7. The bar gene, which codes for the production of PAT, is derived from ACS-GHØØ1-3. This enzyme renders glufosinate ammonium inactive in the plant. The expression of the bar gene allows the plant to survive the otherwise lethal application of glufosinate ammonium. The bar gene was isolated from Streptomyces hygroscopius, a gram-positive soil bacterium. The novel traits of each parental line have been combined, through traditional plant breeding, to produce this new cotton line.

For a full description of each parental line please refer to the individual descriptions for the parent organisms.

This plant is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment.
Other relevant website address or attached documents

Records referencing this document (11)
IDDescription
11record(s) found
Country's Decision or any other Communication4 records
Information Resource1 record
Modified Organism1 record
Organization1 record
Risk Assessment4 records