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Modified Organism
CUH-CP631-7 - Papaya resistant to papaya ringspot virus
Record information and status
Record ID
40296
Status
Published
Date of creation
2007-08-15 11:43 UTC (giovanni.ferraiolo@cbd.int)
Date of last update
2014-01-17 19:36 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-01-17 19:36 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Papaya resistant to papaya ringspot virus
Transformation event
63-1
Unique identifier
CUH-CP631-7
Developer(s)
Cornell University
United States of America
Description
Papaya lines 63-1 was developed using recombinant DNA techniques to resist infection by papaya ringspot virus (PRSV), a major limiting factor in papaya production.

This papaya line was developed by inserting virus-derived sequences that encode the PRSV coat protein (CP). The introduced viral sequences do not result in the formation of any infectious particles, nor does their expression result in any disease pathology.

PRSV belongs to the potyvirus group and is an aphid-transmissible RNA virus that commonly infects papaya, causing serious disease and economic loss.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Carica papaya - Papaya, Pawpaw, Papaw, CARPA
Point of collection or acquisition of the recipient organism
Cultivar: Sunset
Related LMOs
CUH-CP551-8 - Papaya resistant to papaya ringspot virus
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Papaya ringspot potyvirus resistance Selectable marker genes and reporter genes
Show detection method(s)
Characteristics of the transformation process
Vector
pGA482GG/cpPRV-4
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
CaMV 35S promoter
0.53 Kb
 
 
PRSV coat protein
0.92 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
 
Nopaline Synthase Gene Promoter
0.18 Kb
 
 
Neomycin Phosphotransferase II
0.82 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The Agrobacterium tumefaciens binary plasmid pGA482GG/cpPRSV-4 used for the transformation contained three plant-expressible genes, the PRSV CP, neo, and uidA genes. The plasmid also had two genes encoding resistance to tetracycline and gentamycin antibiotics, respectively, but their associated DNA regulatory sequences enabled expression only in bacteria. The plasmid included the right- and left-border regions derived from the A. tumefaciens T-DNA.

Expression of the PRSV CP gene was controlled by including promoter and transcription termination and polyadenylation signal sequences derived from the 35S transcript of cauliflower mosaic virus (CaMV).

In addition, the CP gene sequences were fused to the 5' untranslated sequence and the first 39 nucleotides from the cucumber mosaic virus (CMV) CP to enhance translation of the transgene mRNA. The inclusion of these additional sequences was necessary because PRSV naturally encodes its CP as part of a polyprotein and, therefore, the CP coding region normally lacks a translation initiation ATG codon.

Expression of the neo gene was under control of the promoter and terminator sequences from the nopaline synthase (nos) gene of A. tumefaciens. The second marker gene, uidA, was modified for plant expression by the addition of 35S promoter region from CaMV and the nos 3'-termination region.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Food
Detection method(s)
Additional information
Southern blot analyses indicated that line 63-1 contained intact, functional genes encoding the PRSV CP and NPTII, and did not contain the GUS encoding gene. Genomic hybridization with probes to the gentamycin resistance gene and the Ori T/Tet region indicated that either all or part of the genes for gentamycin and tetracycline resistance had integrated into the papaya genome. However, these genes were not functional since their bacterial promoters cannot drive expression of these genes in plants.

Records referencing this document (3)
IDDescription
3record(s) found
Country's Decision or any other Communication1 record
Modified Organism1 record
Organization1 record