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Modified Organism
Chicory modified for increased inulin expression
Record information and status
Record ID
40313
Status
Published
Date of creation
2007-08-16 15:45 UTC (bch@cbd.int)
Date of last update
2012-09-06 14:18 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-09-06 14:18 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Chicory modified for increased inulin expression
Transformation event
Not available
Developer(s)
Plant Research International
Dept. Genetics and Breeding
Description
The aim of the genetic modification is to reinforce the SST activity naturally present in the chicory root. Besides this main trait the transformed chicory also expresses the nptII gene resulting in resistance to the antibiotic kanamycin.

It is expected that this extra SST will compensate for the decrease of activity of the native SST. This compensation should result in the maintenance, or even increase, of the mean degree of polymerisation of the inulin in the transgenic chicory roots. After a frost period fructan exohydrolase (FEH)is expressed in chicory's roots. FEH catalyses the depolymerization of fructan, decreasing the value of the extracted fructan at harvest. It is expected that the extra SST introduced in the GM chicory will contribute to the re-synthesis of fructan after a frost period.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Cichorium intybus - Chicory
Point of collection or acquisition of the recipient organism
Cultivar/ Breeding line: 95/9 and 145/1
Characteristics of the transformation process
Vector
pSST331 and pA33236 (derived from pBINPLUS)
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
5' Untranslated Leader of AMV RNA4
0.00 Kb
 
 
Sucrose:sucrose 1-fructosyl transferase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Inserted DNA
Two vectors were constructed for transformation each with a diffrent version of the Sucrose, sucrose fructosyltransferase gene as indicated:
T-DNA from pSST331 - sst103: Sucrose, sucrose fructosyltransferase from Helianthus tuberosus;
T-DNA from pA33236: -  a33: Sucrose, sucrose fructosyltransferase from Helianthus tuberosus;
LMO characteristics
Modified traits
  • Increased inulin
Common use(s)
  • Food
Additional Information
Other relevant website address or attached documents

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