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Modified Organism
Potato with modified starch composition
Record information and status
Record ID
40314
Status
Published
Date of creation
2007-08-16 15:46 UTC (bch@cbd.int)
Date of last update
2013-09-18 13:55 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-09-18 13:55 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato with modified starch composition
Transformation event
AM02-1003, AM02-1005, AM02-1012, AM02-1017 and AM99-1089.
Developer(s)
BASF Plant Science GmbH
Carl-Bosch-Str. 38
Ludwigshafen
Germany, 67056
Phone:+49 621 60-0
Fax:+49 621 60-42525
Email:global.info@basf.com
Url:BASF AG - Pflanzenbiotechnologie
Description
A gene modifying the starch composition in potato tubers raising the level of amylopectin has been introduced into potato. The neomycin phophotransferase II gene or an acetohydroxyacid synthase gene have been used as selectable marker genes.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Potato variety Seresta (lines AM02-1003, AM01-1005, AM02-1012) and Kuras (line AM02-1017)
Characteristics of the transformation process
Vector
Derived from pZPZ200 for construct pAP2 and pGPTV-KAN for construct pHAxwO
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Granule bound starch synthase gene promoter
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Granule-bound starch synthase gene
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Nopaline Synthase Gene Terminator
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Nopaline Synthase Gene Promoter
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Acetohydroxy acid synthase gene
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Nopaline Synthase Gene Terminator
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Nopaline Synthase Gene Promoter
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Neomycin Phosphotransferase II
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Transcript 7 gene 3' untranslated region
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
Inserted DNA
a) A gene containing the coding region of potato gbss in antisense orientation relative to the promoter, flanked by the the gbss promoter from Solanum tuberosum and the polyadenylation sequence from Agrobacterium tumefaciens nopaline synthase gene has been inserted into potato variety Seresta (lines AM02-1003, AM01-1005, AM02-1012) and Kuras (line AM02-1017) thus reducing the amount of amylose in the starch fraction. An ahas gene (acetohydroxyacid synthase) from Arabidopsis thaliana flanked by the Agrobacterium tumefaciens nos gene promoter and the octopine synthase polyadenylation sequence serves as selectable marker gene conferring tolerance to Imazamox.

b) AM99-1089 serves as a reference line. The inserted gene consists of the potato gbss (granule bound starch synthase) promoter, the coding region of potato gbss in antisense orientation and the polyadenylation sequence from Agrobacterium tumefaciens nopaline synthase gene thus reducing the amount of amylose in the starch fraction. In addition the neomycin phosphotransferase gene (nptII) connected to the Agrobacterium tumefaciens nopaline synthase promoter and g7 polyadenylation sequence from Agrobacterium tumefaciens has been inserted as selectable marker gene conferring resistance to kanamycin.

LMO characteristics
Modified traits
Common use(s)
  • Food

Records referencing this document (4)
IDDescription
4record(s) found
Modified Organism3 records
Risk Assessment1 record