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Modified Organism
Potato with altered starch composition
Record information and status
Record ID
40315
Status
Published
Date of creation
2007-08-16 15:46 UTC (bch@cbd.int)
Date of last update
2012-10-30 17:14 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-10-30 17:14 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato with altered starch composition
Transformation event
AM99-2003
Developer(s)
BASF Plant Science GmbH
Url:BASF Plant Science
Description
Fragments of the genes of the starch branching enzymes, be1 and be2, were introduced to modify the starch composition in potato tubers, thus raising the level of amylose.

Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Cultivar/breeding line: Dinamo
Characteristics of the transformation process
Vector
Derived from pGTV-KAN
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Granule bound starch synthase gene promoter - Solanum tuberosum - Potato, SOLTU
Branching Enzyme 1 - Solanum tuberosum - Potato, SOLTU
altered carbohydrate composition: increased amylopectin content
Branching Enzyme 2 - Solanum tuberosum - Potato, SOLTU
altered carbohydrate composition: increased amylopectin content
Nopaline Synthase Gene Terminator - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
Nopaline Synthase Gene Promoter - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
Neomycin Phosphotransferase II - Escherichia coli - ECOLX
Resistance to antibiotics - Kanamycin
Transcript 7 gene 3' untranslated region - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
Notes regarding the genetic elements introduced or modified in this LMO
In potato line AM99-2003 a gene consisting of gbss promoter from Solanum tuberosum, the coding region fragments of be1 and be2 (starch-branching enzyme) in tandem and antisense orientation relative to the promoter and the nos polyadenylation sequence from Agrobacterium tumefaciens have been inserted into potato variety Dinamo thus reducing the amount of amylopectin in the starch fraction of the tuber.

In addition the neomycin phosphotransferase gene (nptII) connected to the Agrobacterium tumefaciens nopaline synthase promoter and g7 polyadenylation sequence from Agrobacterium tumefaciens has been inserted as selectable marker gene conferring resistance to kanamycin.
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Other relevant website address or attached documents

Records referencing this document (3)
IDDescription
3record(s) found
Modified Organism1 record
Risk Assessment2 records