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Modified Organism
Non flowering apple tree, modified for resistance to fungi
Record information and status
Record ID
40317
Status
Published
Date of creation
2007-08-16 15:47 UTC (bch@cbd.int)
Date of last update
2012-09-06 14:31 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-09-06 14:31 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Non flowering apple tree, modified for resistance to fungi
Transformation event
Multiple events
Developer(s)
Plant Research International
Dept. Genetics and Breeding
Wageningen
Netherlands
Description
The genetically modified apple trees are inoculated onto non-modified rootstocks. The GM apples carry a kanamycin resistance gene nptII, a modified beta-glucuronidase gene (GUS-intron) and a hth gene encoding a type 1 alpha-hordothionine precursor conferring resistance to fungi.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Malus domestica - Apple, MALDO
Characteristics of the transformation process
Vector
pMOG402.hth.gusintron
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Neomycin Phosphotransferase II - Escherichia coli - ECOLX
Resistance to antibiotics - Kanamycin
Alpha-hordothionine gene - Hordeum vulgare - Barley, HORVU
Resistance to diseases and pests - Fungi
Beta-Glucuronidase coding sequence - Escherichia coli - ECOLX
Selectable marker genes and reporter genes
Nopaline Synthase Gene Promoter - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
Nopaline Synthase Gene Terminator - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
CaMV 35S promoter - Cauliflower mosaic virus - CaMV
CaMV 35S terminator - Cauliflower mosaic virus - CaMV
Notes regarding the genetic elements introduced or modified in this LMO
Additional information on the inserted DNA sequences
The binary vector pMOG402.hth.gusintron contains between the borders the following elements:
- gus(int): B-glucuronidase gene, besides original version also a version with an intron inserted which originates from plants

Vector information
The binary vector pMOG402.hth.gusintron is a derived from pBIN19. It can be propagated both in E. coli as well as A. tumefaciens.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Food
Additional Information
Other relevant website address or attached documents

Records referencing this document (2)
IDDescription
2record(s) found
Modified Organism1 record
Risk Assessment1 record