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Gene and DNA Sequence
Record information and status
Record ID
43634
Status
Published
Date of creation
2008-01-14 17:52 UTC (manoela.miranda@cbd.int)
Date of last update
2013-03-22 20:01 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-03-22 20:01 UTC (dina.abdelhakim@cbd.int)

General information
Name of the gene or DNA sequence
mCry3A
Abbreviation
CS-mCry3A
Category of DNA sequence
  • Protein coding sequence
Is the gene / DNA sequence is a synthetic molecule?
Yes
Donor organism
Donor organism
Bacillus thuringiensis - Bt, Bacillus, BACTU
Donor organism point of collection
Subspecies: tenebrionis
Characteristics of the protein coding sequence
Name of the protein expressed by the coding sequence
mCry3A delta-endotoxin
Biological function
mCry3A delta-endotoxin is a synthetic, maize optimized, modified cry3A (mcry3A) gene whose expression produces a mCry3A insect control protein that is a member of a class of proteins which occur naturally in the gram-positive soil bacterium Bacillus thuringiensis subsp. tenebrionis.

Additional changes in this maize-optimized gene were made, such that the mCry3A protein has enhanced activity against the western corn rootworm and other related pests: (1) its N-terminus corresponds to methionine-48 of the native protein and (2) a cathepsin-G protease recognition site has been introduced, beginning at amino acid residue 155 of the native protein. This cathepsin-G recognition site has the sequence alanine-alanine-proline-phenylalanine, and has replaced the amino acids valine-155, serine-156, and serine-157 in the native protein. The consensus recognition site for cathepsin-G was determined to be alanine-alanine-proline-phenylalanine.
Related trait(s) or use(s) in biotechnology

Records referencing this document (209)
IDDescription
209record(s) found
Country's Decision or any other Communication90 records
Modified Organism27 records
Risk Assessment92 records