Loading...
You are viewing a DELETED record.
This record information is displayed for reference purpose only and should be not used.
This document has been updated. This is not the latest published version. Click here to view the latest version of the record.
Genetic element
(GENE)
published: 18 Jul 2008
last updated: 24 Feb 2014
Beta-galactosidase gene
EN
CS-betagal-ECOLI
EN
Protein coding sequence
No
-
BCH-ORGA-SCBD-14930-5 Organism Escherichia coli (ECOLX)Bacteria
EN
ß-Galactosidase
EN
β-galactosidase is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins.
It is commonly used in molecular biology as a reporter marker to monitor gene expression. It also exhibits a phenomenon called α-complementation which forms the basis for the blue/white screening of recombinant clones. This enzyme can be split in two peptides, LacZα and LacZΩ, neither of which is active by itself but when both are present together, spontaneously reassemble into a functional enzyme.
EN
It is commonly used in molecular biology as a reporter marker to monitor gene expression. It also exhibits a phenomenon called α-complementation which forms the basis for the blue/white screening of recombinant clones. This enzyme can be split in two peptides, LacZα and LacZΩ, neither of which is active by itself but when both are present together, spontaneously reassemble into a functional enzyme.
EN
Record type | Field | Record(s) | |||||
---|---|---|---|---|---|---|---|
|