The strain NVRmp 05/33 is a mutant of NZ05/33. It has only been
modified in the rmp gene which has been truncated and replaced with
an erythromycin antibiotic cassette prepared by transforming the
parent strain NZ05/33 strain with an appropriate plasmid
pBsΔrmpERM. The knockout mutant consists of the upstream and
downstream homologous flanking regions of the rmp gene with the
erythromycin antibiotic cassette sequence inserted in between these
regions ie the gene that encodes the protein determining
erythromycin resistance [4]. The result is a bacterium where most
of the rmp gene has been deleted. This mutant is named NVRmp- 05/33
and has been made at the Novartis Vaccines, Siena, Italy.
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