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Modified Organism
ACS-BNØØ9-3 - Liberator™ canola
Record information and status
Record ID
46388
Status
Published
Date of creation
2008-08-18 15:27 UTC (manoela.miranda@cbd.int)
Date of last update
2012-09-14 13:09 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-09-14 13:09 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Liberator™ canola
Transformation event
pHoe6/Ac
Unique identifier
ACS-BNØØ9-3
Developer(s)
Bayer CropScience
Description
The rapeseed line pHoe6/Ac was genetically engineered to express tolerance to glufosinate ammonium, the active ingredient in phosphinothricin herbicides (Basta®, Rely®, Finale®, and Liberty®).

Glufosinate tolerance in pHoe6/Ac rapeseed is the result of introducing a synthetic copy of the gene encoding the enzyme phosphinothricin-N-acetyltransferase (PAT) isolated from the common aerobic soil actinomycete, Streptomyces viridochromogenes, the same organism from which glufosinate was originally isolated. The PAT enzyme catalyzes the acetylation of phosphinothricin, detoxifying it into an inactive compound. The PAT enzyme is not known to have any toxic properties.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Related LMOs
ACS-BNØ1Ø-4 - Falcon™ rapeseed
Resistance to herbicides - Glufosinate
Show detection method(s)
Characteristics of the transformation process
Vector
pHoe6/Ac
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ti plasmid right border repeat
0.03 Kb
 
 
CaMV 35S promoter
0.53 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.21 Kb
 
 
Ti plasmid left border repeat
0.02 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Canola with tolerance to the herbicide phosphinothricin (Glufosinate ammonium) conferred through insertion of a copy of the phosphinothricin acetyltransferase (pat) gene from the aerobic actinomycete Streptomyces viridochromogenes. Since the native pat gene has a high G:C content, which is atypical for plants, a modified nucleotide sequence was synthesised using codons preferred by plants. The amino acid sequence remains unchanged.

LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Detection method(s)
Additional information
DNA-based methods:
DNA-based methods available include PCR and Southern-Blot methodology. They allow detection and identification of the event Liberator pHoe6/Ac through detection of nucleotide sequences that are specific to these events.
Protein-based methods:
Protein-based methods available include quantitative methods (e.g. specific PAT protein ELISA test) or qualitative methods (e.g. Trait LL Leaf Test kit) based on the specific interaction between antibodies and the PAT protein produced by the introduced gene. They allow detection and identification of glufosinate-ammonium herbicide tolerance trait through detection of the PAT protein in the product. Protein-based methodology offers an easier-to-use alternative to DNA-based methodology.

In addition to these methods, control samples of the product genetic material are available.
Additional Information
Additional Information
Glufosinate chemically resembles the amino acid glutamate and acts to inhibit an enzyme, called glutamine synthetase, which is involved in the synthesis of glutamine. Essentially, glufosinate acts enough like glutamate, the molecule used by glutamine synthetase to make glutamine, that it blocks the enzyme's usual activity. Glutamine synthetase is also involved in ammonia detoxification. The action of glufosinate results in reduced glutamine levels and a corresponding increase in concentrations of ammonia in plant tissues, leading to cell membrane disruption and cessation of photosynthesis resulting in plant withering and death.
Other relevant website address or attached documents

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication1 record
Modified Organism1 record
Organization1 record
Risk Assessment1 record