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Modified Organism
Black Nightshade modified for reduced jasmonate synthesis and antibiotic resistance
Record information and status
Record ID
48032
Status
Published
Date of creation
2009-01-08 12:58 UTC (manoela.miranda@cbd.int)
Date of last update
2012-09-14 15:47 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-09-14 15:47 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Black Nightshade modified for reduced jasmonate synthesis and antibiotic resistance
Transformation event
S04-74
Developer(s)
Max-Planck-Institut für Chemische Ökologie
Hans-Knöll-Strasse 8
D-07745 Jena
Germany
Description
In these plants, lipoxygenase 3 synthesis was reduced with the aim of examining the effects of lowering the jasmonate content in the plants in this way on further interaction processes between the plant and the environment.

The native Solanum nigrum gene SnLOX3 (S. nigrum lipoxygenase 3) codes for a jasmonic acid biosynthesis enzyme. Jasmonates are known plant signalling substances. They play an important role in development processes (maturation of pollen, fruit and seed) and also in plant reactions to biotic and abiotic stress factors, including the production of antibodies for the direct and indirect defence of plants against chewing insects. Constitutive reduction of the lipoxygenase 3 contents can influence one of the insect-induced plant defence cascades, it can alter the defence response of the plant to abiotic and other biotic stress factors, and it can affect the development processes of the plant in general.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum nigrum - Black nightshade, SOLNI
Point of collection or acquisition of the recipient organism
Cultivar/breeding line: Sn30
Related LMOs
Black Nightshade modified for antibiotic resistance
Resistance to antibiotics - Hygromycin Selectable marker genes and reporter genes
Black Nightshade modified for reduced jasmonate synthesis and antibiotic resistance
Resistance to antibiotics - Hygromycin Resistance to chewing insects Selectable marker genes and reporter genes
Characteristics of the transformation process
Vector
pSOL3SLOX
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ti plasmid right border repeat
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
 
Lipoxygenase 3
0.31 Kb
 
 
Pyruvate orthophosphate dikinase, Intron 3
0.79 Kb
 
 
Lipoxygenase 3
0.31 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Hygromycin B phosphotransferase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Ti plasmid left border repeat
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
For the purpose of reducing lipoxygenase 3 synthesis, a construct was developed in which two complementary, internal fragments of the SnLOX3 gene were arranged in sense and in antisense orientation, separated by a spacer and transformed back into S. nigrum plants. These fragments induce RNA-silencing, which reduces the steady-state levels of SnLOX3 mRNA in the transgenic plants, thus reducing the production of the SnLOX3 protein.

Silencing has been engineered and is effected by constitutive expression (enabled by the CaMV 35S promoter) of an antisense-intron-sense gene cassette. Briefly, the mechanism for gene silencing in this GMO include: (1) splicing of the intron 3 from the pyruvate-orthophosphate-dikinase gene (pdk i3) from Flaveria trinervia; (2) hybridization of the RNA of the two SnLOX3 fragments (sense and anti-sense) forming double-stranded RNA molecules (which are unstable in the cells); and (3) degradation of the double-stranded RNA molecules leading to the so-called post-transcriptional gene silencing which is expected to limit the formation of endogenous lipoxygenase 3 and, therewith, the formation of jasmonate.

Agrobacterium tumefaciens was used to insert T-DNA into plant nuclei. A hygromycin resistance gene aph4 from Escherichia coli under the control of the Pnos promoter was utilized to select for transgenic plants. This transgenic genotype bears one copy of the T-DNA and does not contain complete sequences of the nptIII gene.

There were no previous modifications of these plants.
LMO characteristics
Modified traits
  • Resistance to chewing insects
Common use(s)
  • Ornamental
Additional Information
Additional Information
LMO authorized for a specific field trial in Germany (6786-01-0193)
Other relevant website address or attached documents

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication1 record
Modified Organism2 records
Risk Assessment1 record