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Modified Organism
Wheat modified for resistance against corn smut (KP4-Greina 16)
Record information and status
Record ID
48078
Status
Published
Date of creation
2009-01-09 13:33 UTC (manoela.miranda@cbd.int)
Date of last update
2013-09-13 18:04 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-09-13 18:04 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Wheat modified for resistance against corn smut (KP4-Greina 16)
Transformation event
KP4-Greina 16
Developer(s)
Dr. Christof Sautter
Institute of Plant Sciences
Swiss Federal Institute of Technology Zürich (ETH)
Universitätstrasse 2
8092 Zürich
SWITZERLAND
Phone:+41-44-632-5713
Fax:+41-44-632-1044
Email:christof.sautter@ipw.biol.ethz.ch
Url:ETH Zurich
Description
The kp4 gene is derived from the genome of a double-stranded RNA virus (Ustilago Maydis Virus 4, UmV4), which is present in the tissue of certain fungal strains of corn smut (Ustilago maydis). The expression of the kp4 gene (KP4, killer protein 4) in Ustilago maydis cells leads to a reversible inhibition of hyphal growth, but does not kill off competing strains. It is expected that plants expressing the KP4 protein are less susceptible to damage caused by the phytopathogenic fungi Ustilago species. Expression of the kp4 gene is under control of the ubiquitin promoter of the uidA gene from Zea mays and the t35S terminator of CaMV.

The bar gene derives from Streptomyces hygroscopicus and confers herbicide resistance against phosphinothricine (Bialaphos). The gene was used as a transformation marker. Expression of the bar gene is controlled by the actin promoter of Oryza sativa and the t35S terminator of CaMV.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Triticum aestivum - Wheat
Point of collection or acquisition of the recipient organism
Wheat Cultivar/breeding line Greina
Characteristics of the transformation process
Vector
pUbi::kp4 (pUC19 derivate) and pAct::bar (pUC19 derivate) 
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
Rice actin 1 gene promoter
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Phosphinothricin N-acetyltransferase gene
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CaMV 35S terminator
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Ubiquitin gene promoter
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Killer Protein 4
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CaMV 35S terminator
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Further details
LMO characteristics
Modified traits
Common use(s)
  • Food

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record