Observed gene expression in the transgenic plants
In the genetically modified lines a low level of expression of both
Rpi-blb1 and Rpi-blb2 has been demonstrated by real-time PCR
analysis in leaves, stems, tubers and roots. In flowers a low
expression of Rpi-blb2 can be detected, while Rpi-blb1 is expressed
at even lower levels or not at all.
The ahas gene is controlled by the nos promoter, which is known to
result in low expression levels in all parts of the plant.
Expression of the ahas gene in the genetically modified potato
material has been demonstrated in tissue culture by survival on
media containing Imazamox.