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Modified Organism
Potato modified for resistance to Phytophthora infestans
Record information and status
Record ID
48362
Status
Published
Date of creation
2009-03-05 12:50 UTC (manoela.miranda@cbd.int)
Date of last update
2012-08-15 20:47 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-15 20:47 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato modified for resistance to Phytophthora infestans
Transformation event
257 lines
Developer(s)
BASF Plant Science GmbH
Carl-Bosch-Str. 38
Ludwigshafen
Germany, 67056
Phone:+49 621 60-0
Fax:+49 621 60-42525
Email:global.info@basf.com
Url:BASF AG - Pflanzenbiotechnologie
Description
The Rpi-blb1 and Rpi-blb2 genes inserted in these genetically modified potatoes should confer increased resistance to Phytophthora infestans. Rpi-blb1 and Rpi-blb2 code for proteins of the NBS-LRR (nucleotide binding site-leucine rich repeat) type.

The ahas gene inserted in the potato plants imparts tolerance to the herbicidal active substance Imazamox to the shoots during the selection process in cell culture.

Plasmid DNA was introduced into the potato lines by Agrobacterium-mediated gene transfer technology.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Characteristics of the transformation process
Vector
Vector VCPMA16 derived from pPZP200
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Phytophthora infestans Resistance gene 2 Promoter
1.53 Kb
 
 
Phytophthora infestans Resistance gene 2
3.89 Kb
 
 
Phytophthora infestans Resistance gene 2 Terminator
2.53 Kb
 
 
Phytophthora infestans Resistance gene 1 Promoter
1.17 Kb
 
 
Phytophthora infestans Resistance gene 1
3.59 Kb
 
 
Phytophthora infestans Resistance gene 1 Terminator
0.41 Kb
 
 
Nopaline Synthase Gene Promoter
0.29 Kb
 
 
Acetohydroxy acid synthase gene
2.01 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
LMO characteristics
Modified traits
Common use(s)
  • Food
Detection method(s)
Additional information
These potato lines contain the T-DNAs in the plasmids VCPMA16 harbouring two genes for improving resistance to Phytophthora infestans (Rpi-blb1 and Rpi-blb2) as well as an acetohydroxyacid synthase (ahas) gene for tolerance to herbicide.

Low level of expression of both Rpi-blb1 and Rpi-blb2 was detected by real-time PCR analysis in leaves, stems, tubers and roots. In flowers a low expression of Rpi-blb2 was detected, while Rpi-blb1 is expressed at even lower levels or not at all.

The At-ahas gene with a point mutation is controlled by the nos promoter, which is known to result in low expression levels in all parts of the plant. Expression of the ahas gene in the genetically modified potato material has been demonstrated in tissue culture by survival on media containing Imazamox.
Additional Information
Additional Information
Vector information
Derivate of pPZP200. Reference: Hajdukiewicz et al (1994) Plant Mol. Biol., 25, 989-994.
Other relevant website address or attached documents

Records referencing this document (7)
IDDescription
7record(s) found
Country's Decision or any other Communication3 records
Modified Organism1 record
Risk Assessment3 records