Opening of Discussion
POSTED ON BEHALF OF LIJO JOHN
Dear Participants of the Forum,
I am pleased to welcome you to the second round of online discussions of Asia-Pacific workshop on the detection and identification of LMOs.
As agreed at the workshop, and outlined in the report of the meeting the Secretariat is organising a series of online discussions for the group to facilitate the consolidation of the knowledge gained during the workshop and encourage the sharing of information between labs within the region.
It is my privilege to moderate this discussion on the topic of extraction methods. As discussed during the workshop this topic will focus on this discussion will focus on the compilation of information and best practices on the extraction of DNA from difficult matrices.
I would like to invite you to post any relevant material on the matter as well as pose questions and comments on specific areas where additional information is needed so that others that may have this information available can share it with the group.
The discussion will take place for the next two weeks. Your views and feedback will be greatly appreciated. If possible, please post your comments during the early days of the discussion to foster a more lively debate building on the comments of one another.
I look forward to your comments and suggestions!
posted on 2017-07-03 01:14 UTC by Dina Abdelhakim, SCBD
I would like to invite all of you to take part actively in the current online discussion on the topic on DNA extraction from difficult matrices. I believe, this is one of the important in which many of us may be finding difficulties while handling various kinds of products in our lab for DNA extraction.
Kindly take this opportunity to share the knowledge through which we all can improve our own laboratories technical competency.
DNA extraction from honey and oil samples are of two important matrices on which we are interested to develop effective DNA extraction methods which we are finding difficulties. Any one of the participants who is working on to these products may kindly share your experiences/information which can help.
This discussion will be continuing for another one week. Kindly post your views and feedback which will be highly appreciated. On coming days I will try to share few reference which can help.
Once again I request all the participants to be more active on to this forum.
posted on 2017-07-05 14:50 UTC by Dr Lijo John, Export Inspection Council Of India
Dear Dr. Lijo, thank you for giving us of your time to moderate this forum, i believe most of the participants have a busy agenda, however, let's try to be more active through these forums and share our experiences and needs for improvements. I personally do not work on oil and honey samples, however i would be very interested if someone shares his experiences. It would be also interesting to me if someone share his experience with seeds, the techniques to remove polysaccharides and poly-phenol compounds during the DNA extraction, what are the reagents that are specifically applied to remove these contaminants which are usually co-extracted with the DNA. In my laboratory we use the CTAB extraction protocol, since it is approved by EURL-GMFF and it has shown to be efficient to extract DNA from different matrices, including highly processed samples.
posted on 2017-07-05 18:24 UTC by Mr. Joe Said, Lebanon
Dear Mr. Joe, thank you for your message. In our laboratory also mainly we are following CTAB based extraction protocol. We follow column purification methods as well. In many cases, extraction using CTAB buffer followed spin column purification giving better results w.r.t purity of DNA.
posted on 2017-07-06 10:11 UTC by Dr Lijo John, Export Inspection Council Of India
Dear participants, I would like to share few research papers downloaded in which DNA extraction from oil and honey samples were done. Hope this will be of use. We also tried in these lines and could get some results, but sometimes very less or no yield or low purity. I request you to share your experiences or information which can help other participants.
Kindly do participate in the discussion and try to contribute something.
(edited on 2017-07-07 11:37 UTC by Dr Lijo John)
posted on 2017-07-07 11:35 UTC by Dr Lijo John, Export Inspection Council Of India