Volume 245, Issues 1–2, 13 March 1989, Pages 51–56
Nucleotide sequence and organization of potato leafroll virus genomic RNA
- a Agricultural University Wageningen, Department of Virology, PO Box 8045, 6700 EM Wageningen, The Netherlands
- b Research Institute for Plant Protection, PO Box 9060, 6700 GW Wageningen The Netherland
- c MOGEN International NV, Einsteinweg 97, 2333 CB Leiden, The Netherlands
- Received 18 January 1989. Available online 23 October 2001.
Abstract
The nucleotide sequence of the genomic RNA of potato leafroll virus was determined and its genetic organization deduced. The RNA is 5882 nucleotides long and contains 6 open reading frames (ORFs) encoding proteins of 70, 70, 56, 28, 23 and 17 kDa. The putative genes for the coat protein (23 kDa) and the RNA-dependent RNA polymerase (70 kDa) were identified by interviral amino acid sequence homologies. For expression of the different ORFS, translational frameshift and readthrough events are proposed.
Keywords
- cDNA cloning;
- Nucleotide sequence;
- Amino acid sequence homology;
- RNA virus, plant positive-strand;
- (Potato leafroll virus)
Abbreviations
- BWYV, beet western yellows virus;
- BYDV, barley yellow dwarf virus;
- CarMV, carnation mottle virus;
- CPMV, cowpea mosaic virus;
- ORF, open reading frame;
- PLRV, potato leafroll virus
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Copyright © 1989 Published by Elsevier B.V.