Potato modified for increased Stomata Density | BCH-LMO-SCBD-100293 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 20 Jul 2009 last updated: 11 Mar 2013
Living Modified Organism identity
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Potato modified for increased Stomata Density
Lines: 2, 6, 12
  • - Organization: Max Planck Institute of Molecular Plant Physiology (MPIMP) | BCH-CON-DE-49374-2
    Max Planck Institute of Molecular Plant Physiology (MPIMP)
    , Max-Planck-Institut für Molekulare Pflanzenphysiologie
    Am Mühlenberg 1
    14476 , Germany
    Phone: +49 331 567 - 80 ,
    Fax: +49 331 567 - 84 08,
Transgenic potatoes expressing an RNAi construct against the endogenous StSDD1 gene. The resulting plant exhibits an increase in stomata density by up to 25%. Thereby these plants have enhanced transpiration activity, decreased leaf roll at high light intensities, and increased tuber yield.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar/breeding line: Désirée
  • Potato modified for decreased Stomata Density
    | Max-Planck-Institute of Molecular Plant Physiology | Resistance to antibiotics (Hygromycin), Tolerance to abiotic stress (Drought)
Characteristics of the modification process
pBinAR-StSDD1 (Derived from pBIN19)
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Transgenic potatoes pBINAR-StSDD1 contain the StSDD1 RNAi construct consisting of the CaMV 35S promoter, a fragment of the endogenous StSDD1 gene in sense and antisense orientation separated by a part of the multiple cloning site of the vector pCR2.1-TOPO and the ocs terminator that mediates increased formation of stomata in leaves. These potatoes further encode an nptII cassette as a selection marker system that confers tolerance to aminoglycoside antibiotics (kanamycin, neomycin, geneticin). Here, the nptII gene is put under the control of the nos promoter and –terminator.
LMO characteristics
  • BCH-GENE-SCBD-48460-3 Stomatal density and distribution 1 gene | (Potato)
    Protein coding sequence | Tolerance to abiotic stress (Drought)
In order to increase guard cell density, the expression of the potato's own SDD1 protein was reduced in these potato plants with the aid of an RNAi construct (pBinAR-StSDD1). Using the RNAi construct, the RNA of a part of the SDD1 gene is transcribed in sense and antisense orientation so that sense and antisense RNA are separated by a sufficiently long spacing sequence. The sense and antisense sections of the RNAi form a RNA doublestrand. The RNA double strand is recognized by specific enzymes in the plant cell and broken down into small fragments. These RNA fragments adhere to the mRNA of the endogenous SDD1 gene and activate the degradation of the mRNA.
  • Research
Detection method(s)
Additional Information
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