BCS-GHØØ3-6 - Herbicide-tolerant, insect-resistant cotton | BCH-LMO-SCBD-100730 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 04 Sep 2009 last updated: 16 Jul 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant, insect-resistant cotton
EN
T303-3
Yes
BCS-GHØØ3-6
LM cotton line, T303-3,was modified for protection against feeding damage by Lepidopteran insect larvae, and tolerance to herbicides containing glufosinate ammonium.

Insect protection is conferred by expression of a modified Cry1Ab protein from Bacillus thuringiensis and herbicide tolerance is conferred by expression of phosphinothricin acetyltransferase (PAT) from Streptomyces hygroscopicus.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Line: Coker 315
EN
  • BCS-GHØØ4-7 - Herbicide-tolerant, insect-resistant cotton
    | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis)), Resistance to herbicides (Glufosinate)
Characteristics of the modification process
pTDL004
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-104947-3 5'e1 Leader | Oryza sativa (Rice, ORYSA)
    Leader
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    BCH-GENE-SCBD-101025-5 NADP-malic enzyme 1 gene 3'UTR and terminator | Flaveria bidentis (Coastal plain yellowtops, FLABI)
    Terminator
Southern blot and sequencing analysis indicated that single intact copies of each expression cassette was integrated into the host genome, however the insert was integrated in a rearranged fashion as compared to the vector sequence.
EN
LMO characteristics
EN
  • Other (Industrial use)
Detection method(s)
EN
Additional Information
EN
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