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Modified Organism
DP-3Ø5423-1 x MON-Ø4Ø32-6 - Modified fatty acid, herbicide-tolerant soybean
Record information and status
Record ID
Date of creation
2010-07-05 18:46 UTC (ralvarez@conacyt.mx)
Date of last update
2019-07-12 19:53 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-07-12 19:53 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Modified fatty acid, herbicide-tolerant soybean
Transformation event
305423 x GTS 40-3-2
Unique identifier
DP-3Ø5423-1 x MON-Ø4Ø32-6
Pioneer Hi-Bred
Pioneer Hi-Bred
United States of America
Phone:(515) 270-3200
The stacked soy line DP-3Ø5423-1 x MON-Ø4Ø32-6 was obtained through the traditional cross breading of each of the parental organisms to produce a soy that expresses each of EPSPS, ALS and omega-6-fatty acid dehydrogenase genes. The expression of these genes are expected to confer tolerance to sulphonylurea and glyphosate herbicides and  increased levels of monounsaturated fatty acid and decreased levels of polyunsaturated fatty acids.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
DP-3Ø5423-1 - TREUS™Plenish™ Soybean
Changes in quality and/or metabolite content - Lipid and fatty acids Resistance to herbicides - Sulfonylurea
Show detection method(s)
MON-Ø4Ø32-6 - Roundup Ready™ soybean
Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
PV-GMGT04, PHP19340 and PHP17752
Techniques used for the modification
  • Cross breeding
Genetic elements construct
Kunitz trypsin inhibitor gene promoter
2.08 Kb
0.60 Kb
Kunitz trypsin inhibitor gene terminator
0.20 Kb
SAMS Promoter
1.30 Kb
Acetohydroxy acid synthase gene
1.97 Kb
Acetohydroxy acid Synthase gene Terminator
0.60 Kb
CaMV Enhanced 35S promoter
0.61 Kb
Chloroplast Transit Peptide 4
0.23 Kb
5-enolpyruvylshikimate-3-phosphate synthase gene
1.36 Kb
Nopaline Synthase Gene Terminator
0.26 Kb
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from GTS 40-3-2 vector PV-GMGT04
The soybean line GTS 40-3-2 was developed to allow for the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option. This genetically engineered soybean line contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows GTS 40-3-2 to survive the otherwise lethal application of glyphosate.

DNA insert from 305423 vector PHP19340 and PHP17752
The intended effect of the modification in 305423 soybean is to produce soybean seeds with increased levels of monounsaturated fatty acid and decreased levels of polyunsaturated fatty acids through the insertion of the microsomal omega-6 desaturase gene (FAD2-1) into soybean. Transcription of the gene fragment acts to silence the expression of the endogenous soybean omega-6 desaturase, which results in an increased level of oleic acid and decreased levels of linoleic and linolenic acids in the soybean seed. The acetolactate synthase (als gene) that confers tolerance to a sulfonylurea herbicide was used as a selectable marker for the transformation.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Other gene(s) whose expression was affected by the transformation
omega-6-desaturase - Glycine max - Soybean, Soya bean, Soya, SOYBN
Changes in quality and/or metabolite content - Lipid and fatty acids
How the expression of the gene(s) was affected
The DNA insert from  PHP19340 and PHP17752 contains a fragment corresponding to 40% of the soy omega-6-desaturase. Upon transcription of the fragment, the resulting transcript acts to silencing the endogenous gene by RNA interference.
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (29)
29record(s) found
Country's Decision or any other Communication8 records
Modified Organism2 records
Organization5 records
Risk Assessment14 records