SYN-E3272-5 × SYN-BTØ11-1 × SYN-IR6Ø4-5 × MON-ØØØ21-9 - Modified thermostable alpha-amylase, insect-resistant, herbicide-tolerant maize | BCH-LMO-SCBD-101287 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 23 Jul 2010 last updated: 12 Feb 2021
Living Modified Organism identity
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Modified thermostable alpha-amylase, insect-resistant, herbicide-tolerant maize
3272 x Bt11 x MIR604 x GA21
SYN-E3272-5 × SYN-BTØ11-1 × SYN-IR6Ø4-5 × MON-ØØØ21-9
The stacked maize line SYN-E3272-5, SYN-BTØ11-1, SYN-IR6Ø4-5 and MON-ØØØ21-9 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of amy797E alpha amylase, PMI, Cry1Ab, PAT, mCry3A and EPSPS genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to glufosinate and glyphosate herbicide as well as the synthesis of thermostable alpha-amylase.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)
  • BCH-LMO-SCBD-15109-9 Living Modified Organism SYN-E3272-5 - Enogen™ Maize
    Use in industrial applications (Biofuel production)
  • BCH-LMO-SCBD-14797-15 Living Modified Organism SYN-BTØ11-1 - YieldGard™ maize
    Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis)), Resistance to herbicides (Glufosinate)
  • BCH-LMO-SCBD-15105-12 Living Modified Organism SYN-IR6Ø4-5 - Agrisure™ RW Rootworm-Protected maize
    Resistance to diseases and pests (Insects, Coleoptera (beetles))
  • BCH-LMO-SCBD-14794-18 Living Modified Organism MON-ØØØ21-9 - Roundup Ready™ maize
    Monsanto | Resistance to herbicides (Glyphosate)
  • SYN-BTØ11-1 × SYN-IR6Ø4-5 × MON-ØØØ21-9 - Agrisure™ 3000GT Maize
    | Syngenta Seeds GmbH | Mannose tolerance, Resistance to diseases and pests (Insects, Coleoptera (beetles), Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis)), Resistance to herbicides (Glufosinate, Glyphosate), Selectable marker genes and reporter genes
Characteristics of the modification process
pNOV7013, pZO1502,  pZM26 and pDPG434
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
DNA insert from 3272 vector pNOV7013
Maize containing thermostable alpha-amylase (for optimised bioethanol production) through introduction of the amy797E gene from Thermococcales (thermostable bacterium). The pmi gene expresses the PMI protein, which allows the transformed plants to use mannose as an energy source and is used as a selectable marker.

DNA insert from Bt11 vector pZO1502
Insect-resistant and herbicide tolerant maize produced by inserting the cry1Ab gene to confer resistance to the European corn borer (Ostrinia nubilalis), and the phosphinothricin N-acetyltransferase (PAT) encoding gene to confer tolerance to phosphinothricin (PPT) herbicide, specifically glufosinate ammonium.

DNA insert from MIR604 vector pZM26
MIR604 is a genetically modified to confer field protection against corn root worms. The cry3A gene codes for a Bt-toxin (Cry3A), which confers resistance to coleopteran species. Expression of the pmi gene allows the plant to use mannose as a carbon source through production of the PMI protein, and is used as a selectable marker.

DNA insert from GA21 vector pDPG434
The GA21 line of maize was modified to be tolerant of glyphosate-containing herbicides. The isolated endogenous maize epsps gene was modified through site-directed mutagenesis, such that its encoded enzyme was insensitive to inactivation by glyphosate, and inserted into the inbred AT maize variety.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
  • Food
  • Feed
  • Biofuel
Detection method(s)
Additional Information