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Gene and DNA Sequence
Record information and status
Record ID
Date of creation
2010-12-14 19:18 UTC (manoela.miranda@cbd.int)
Date of last update
2019-12-13 19:59 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-12-13 19:59 UTC (austein.mcloughlin@cbd.int)

General information
Name of the gene or DNA sequence
Tetracycline-controlled transactivator
Category of DNA sequence
  • Protein coding sequence
Is the gene / DNA sequence is a synthetic molecule?
Donor organism
Donor organism
Escherichia coli - ECOLX
Donor organism point of collection
Tetracycline repressor (TetR) from E. coli

VP16 transcriptional activator from HSV-1
Characteristics of the protein coding sequence
Name of the protein expressed by the coding sequence
Tetracycline-controlled transactivator
Biological function
Tetracycline-controlled transactivator (tTAV) is a synthetic protein coding sequence based on a fusion of sequences from Escherichia coli and Herpes simplex virus (VP16 transcriptional activator).

tTAV is under the control of its own binding site, tetO. In the absence of tetracycline, tTAV binds to tetO and drives expression of more tTAV, in a positive feedback loop. In the presence of tetracycline, tTAV binds tetracycline; this tetracycline-bound form does not bind tetO and so does not lead to expression of more tTAV. Consequently, this construct gives very high levels of expression of tTAV in the absence of tetracycline, but only low, basal expression in the presence of tetracycline.

tTAV encodes a dominant trait that, when introduced into certain insects, causes death of the insect unless the antibiotic tetracycline is supplied.
Related trait(s) or use(s) in biotechnology
  • Conditional lethality

Records referencing this document (11)
11record(s) found
Country's Decision or any other Communication3 records
Information Resource1 record
Modified Organism3 records
Risk Assessment4 records