Record details

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Living Modified Organism identity

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LMO name

Brassica napus modified for the synthesis of resveratrol

Transformation event

pPSty5 (line 1104.3.6)

Developer(s)

Record #101524

FINAB e. V.

Vereins zur Förderung Innovativer und Nachhaltiger AgroBiotechnologie Mecklenburg-Vorpommern e. V. (FINAB e. V.)

Thünenplatz 1

Groß Lüsewitz, Mecklenburg-Vorpommern

Germany, 18190

Phone:	+49 (0)381 498 30-81
Email:	webmaster@finab.de
Url:	FINAB e. V.

Description

Brassica napus is modified with the VST1 gene which codes for stilbene synthase. This enzyme catalyses the a step in the reaction that leads to the synthesis of resveratol in Brassica napus.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #12083

Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA

Point of collection or acquisition of the recipient organism

Cultivar/breeding line: Drakkar, Lisora

Characteristics of the transformation process

Vector

pSty5 derived from pPZP111

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

Napin gene promoter

#101521

0.00 Kb

Stilbene Synthase

#101520

0.00 Kb

Stilbene Synthase Terminator

#103887

0.00 Kb

CaMV 35S promoter

#100287

0.00 Kb

Neomycin Phosphotransferase II

#15001

0.00 Kb

CaMV 35S terminator

#100290

0.00 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

The plasmid used to transform the oilseed rape plants with the pPSty5 construct is derived from the binary vector pPZP111 (Hajdukiewicz et al., 1994) and contains the following genetic elements outside the border regions:
- a bacterial chloramphenicol acetyltransferase gene (CmR gene, cat-Gen), which confers resistance to the antibiotic chloramphenicol;
- the bom sequence from pBR322 for mobilisation of the plasmid from E. coli in Agrobacterium tumefaciens;
- the origins of replication from ColE1 and pVS1 for replication in E. coli or Agrobacterium.

LMO characteristics

Modified traits

Changes in quality and/or metabolite content
- Antioxidants
- Flavonoids (e.g. anthocyanin)
Resistance to antibiotics
- Kanamycin
Resistance to diseases and pests
- Fungi

Common use(s)

Research