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Brassica napus modified for the synthesis of resveratrol
FINAB e. V.
Vereins zur Förderung Innovativer und Nachhaltiger AgroBiotechnologie Mecklenburg-Vorpommern e. V. (FINAB e. V.)
Groß Lüsewitz, Mecklenburg-Vorpommern
Brassica napus is modified with the VST1 gene which codes for
stilbene synthase. This enzyme catalyses the a step in the reaction
that leads to the synthesis of resveratol in Brassica napus.
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Cultivar/breeding line: Drakkar, Lisora
pSty5 derived from pPZP111
- Agrobacterium-mediated DNA transfer
Stilbene Synthase Terminator
Neomycin Phosphotransferase II
The plasmid used to transform the oilseed rape plants with the
pPSty5 construct is derived from the binary vector pPZP111
(Hajdukiewicz et al., 1994) and contains the following genetic
elements outside the border regions:
- a bacterial chloramphenicol acetyltransferase gene (CmR gene,
cat-Gen), which confers resistance to the antibiotic
- the bom sequence from pBR322 for mobilisation of the plasmid from
E. coli in Agrobacterium tumefaciens;
- the origins of replication from ColE1 and pVS1 for replication in
E. coli or Agrobacterium.
- Changes in quality and/or metabolite content
- Flavonoids (e.g. anthocyanin)
- Resistance to antibiotics
- Resistance to diseases and pests