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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
High-protein, herbicide resistant wheat HOSUT
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HOSUT
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Organization:Institut für Pflanzengenetik und Kulturpflanzenforschung ()Corrensstrasse 3Gatersleben,
06466 , GermanyPhone: +49 (0)39482 5-0 ,Fax: +49 (0) 39482 5139,Email: info@ipk-gatersleben.de,Website: http://www.ipk-gatersleben.de,
The endosperm-specific expression of the sucrose transporter HvSUT1 in HOSUT wheat leads to increased sucrose uptake into the endosperm that might be used in the amino acid biosynthesis process. Therefore, an increase in the protein content in the wheat grains is expected.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-45396-4 Organism Triticum aestivum (Wheat)Crops
Cultivar/ Breeding line: Certo
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pPZP 200 and pJFBar
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- Biolistic / Particle gun
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-101595-2 Hordein B1 promoter | Hordeum vulgare (Barley, HORVU)Promoter
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BCH-GENE-SCBD-101594-3 Sucrose transporter gene | Hordeum vulgare (Barley, HORVU)Protein coding sequence | Changes in physiology and/or production (Yield),Changes in quality and/or metabolite content (Carbohydrates)
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BCH-GENE-SCBD-101596-4 Hordein B1 terminator | Hordeum vulgare (Barley, HORVU)Terminator
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
By transformation of wheat with the vector pPZP 200 the sucrose transporter HvSUT1 was introduced into the wheat genome. Since HvSUT1 is put under the control of the endopserm-specific hordein promoter B1 and the corresponding hordein terminator, HvSUT1 is exclusively expressed within the endosperm during the phase of increased protein biosynthesis in wheat grains.
This is meant to optimize the carbohydrate supply for increased levels of protein biosynthesis.
Simultaneously, the vector pJFBar was co-transformed to mediate resistance towards phospinotricine (PPT, Glufosinate) to be used as a selective marker system.
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This is meant to optimize the carbohydrate supply for increased levels of protein biosynthesis.
Simultaneously, the vector pJFBar was co-transformed to mediate resistance towards phospinotricine (PPT, Glufosinate) to be used as a selective marker system.
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- Food
- Research
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