Garden Pea modified for the expression of a single chain antibody (scFv) in the seed | BCH-LMO-SCBD-103028 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 25 Jan 2012 last updated: 16 Aug 2012
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Garden Pea modified for the expression of a single chain antibody (scFv) in the seed
EN
BA11
  • - Person: Novoplant GmbH | BCH-CON-SCBD-103027-2
    Person
    Novoplant GmbH
    Am Schwabeplan 1B
    Gatersleben,
    06466, Germany
    Phone: + 49 39482 7998-0,
    Fax: + 49 39482 7998-80,
    Website:
    Related Organization
    Novoplant GmbH ()
    Private sector (business and industry)
    Am Schwabeplan 1B
    Gatersleben,
    06466, Germany
    Phone: + 49 39482 7998-0,
    Fax: + 49 39482 7998-80,
    Website:
BA11-2 pea plants express seed-specific, within the peas, the antibody fragment scFv BA11. This antibody fragment has affinity towards enterotoxigenic Escherichia coli (ETEC) cells that can cause the postweaning diarrhea by adhesion to the intestinal wall in pigs.

To prevent the outbreak of postweaning diarrhea BA11-expressing peas shell be feed to pigs.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Pisum sativum var. Eiffel
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Characteristics of the modification process
pPZP-USP(+)-BA11
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  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
By action of the N-terminal signal peptide LeB4 and the C-terminal KDEL retetion signal the scFv BA11 antibody fragment accumulates within the endoplasmic reticulum but does not enter the secretion pathway. Since the LeB4-scFv BA11-KDEL fusion protein is under the control of the USP(+) promoter seed specific expression is achieved. A N-terminal His-tag allows immuno detection and immunoprecipitation of the fusion protein.
EN
LMO characteristics
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  • Pharmaceutical
Detection method(s)
PCR-based methods, to be developed on information about the transformation vector.
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1