Potato producing vaccine against cholera | BCH-LMO-SCBD-103103 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 20 Feb 2012 last updated: 22 Aug 2012
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Potato producing vaccine against cholera
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potato (Solanum tuberosum) 35SctxBSEK
Expression of the non-toxic B subunit of the cholera toxin . The non-toxic B subunit has been used for more than 20 years as a component of human vaccines against cholera and other diarrheal diseases.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar/ breeding line: desiree, albatros
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Characteristics of the modification process
p35SctxBSEK, derivative of pLH9000
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  • Agrobacterium-mediated DNA transfer
 
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The LMO 35SctxBSEK expresses a synthetic gene of the nontoxic subunit of cholera toxin that corresponds to 71% with the gene sequence of the ctxB gene from Vibrio cholerae (100% amino acid identity). The gene was adapted to the codon preference of higher plants. The ER-retention signal SEKDEL was fused to the cholera toxin subunit in order to stabilize the protein.
In the plant, expression is regulated by the 35S promoter and terminator from the cauliflower mosaic virus, which leads to an expression in all plant parts throughout the entire lifecycle of the potato.
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LMO characteristics
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  • Research
Detection method(s)
PCR-based methods, to be developed on the information about the transformation vector.
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Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1