Insect resistant cotton | BCH-LMO-SCBD-103216 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 20 Mar 2012 last updated: 17 May 2016
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect resistant cotton
This modified cotton contains the insecticidal cry1Ac gene which imparts resistance against Lepidoptera. The LMO also contains the selectable markers nptII to isolate transformed seedlings and a GUS gene cassette as a reporter gene.

Bt-cotton was generated by using the biolistic method of transformation system. The transformed cotton shoots containing the nptII gene were selected on medium supplemented with kanamycin.  A procedure for biolistics method of transformation of cotton is novel and performed using shoot meristem. Plants were regenerated and ultimately plantlets were grown in soil and assayed for insect resistance.

The cry1Ac gene in Indian inbred lines behave as a single dominant Mendelian factor and is stably integrated in the plant genome.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Local collection, India
Characteristics of the modification process
  • Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)
A truncated cry1Ac gene derived from Bacillus thuringiensis subsp. kurstaki (B.t.k.)
LMO characteristics
  • Food
  • Feed
  • Fiber/textile
Detection method(s)
Immunological and DNA based detection methods have been developed for cry1Ac event-1 its expressed protein. ELISA kits from Envirologix, USA are being used for detecting Cry1Ac protein in our laboratory. The gene specific primers designed and supplied by BREF IIT Kharagpur are being used for detecting this event. Central Institute of Cotton Research, ICAR, Nagpur, India, is the referral laboratory for event testing. RT-PCR based LOD (Limit of Detection) protocol was developed and validated at SGS, Ahmedabad, India.     
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 1
Country's Decision or any other Communication Living modified organism(s) 4
Risk Assessment generated by a regulatory process Living modified organism(s) 3