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Insect resistant cotton (MLS 9124)
Dr. K. K. Narayanan
M/s Metahelix Life Sciences Pvt. Ltd
3, KIADB 4th Phase, Bommasandra
Event MLS9124 was developed by transforming cotton plants with a
synthetic gene, cry1C, for the introduction of resistance to
Bt cotton was generated by Agrobacterium mediated transformation of
hypocotyl explants. Transformed events were detected by selection
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Cotton collection from Metahelix
pMH72 (Derivative of pPZP201)
- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Synthetic cry1C gene
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) - armyworm (Spodoptera frugiperda)
Neomycin Phosphotransferase II - Escherichia coli - ECOLX
Resistance to antibiotics - Kanamycin
The synthetic Cry1c gene encodes for a truncated insecticidal
- Resistance to antibiotics
- Resistance to diseases and pests
- Lepidoptera (butterflies and moths)
- Cotton bollworm (Helicoverpa spp.)
Putative transformants were analysed by PCR (Internal Methods),
Southern and ELISA (Envirologix) analysis, and were also assayed
for level of insect resistance.