DAS-Ø15Ø7-1 x MON-ØØ81Ø-6 - Maize modified for insect resistance and herbicide tolerance | BCH-LMO-SCBD-103256 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 27 Mar 2012 last updated: 24 Jul 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Maize modified for insect resistance and herbicide tolerance
EN
TC1507 x MON810
DAS-Ø15Ø7-1 × MON-ØØ81Ø-6
  • - Person: Msc Goran Kuhar Jezovsek | BCH-CON-BR-103245-2
    Person
    Msc Goran Kuhar Jezovsek
    Gerente de Regulamentação e Registros, Regulamentação e Registro
    Du Pont do Brasil S.A. - Divisão Pioneer Sementes Endereço: SGAS 902 Lt 74 Cj B Sala 221 a 224 Bloco A - Ed. Athenas - Asa Sul
    Brasília, Distrito Federal
    70390-020, Brazil
    Phone: 55 - 61 - 21039111,
    Fax: 55 - 61 -21039111,
    Related Organization
    Du Pont do Brasil S.A. (Du Pont - Divisão Pioneer Sementes)
    Private sector (business and industry)
    Du Pont do Brasil S.A. - Divisão Pioneer Sementes Endereço: SGAS 902 Lt 74 Cj B Sala 221 a 224 Bloco A - Ed. Athenas - Asa Sul
    Brasília, Distrito Federal
    70390-020, Brazil
    Phone: 55 - 61 - 21039111,
    Fax: 55 - 61 -21039111,
The stacked maize line was produced through conventional breeding technique of crossing the Herculex I corn event TC1507 with MON810 maize. The cross is insect-resistant maize, tolerant to the herbicide glufosinate ammonium.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)
    Crops
  • BCH-LMO-SCBD-14841-13 Living Modified Organism DAS-Ø15Ø7-1 - Herculex™ I maize
    Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Resistance to herbicides (Glufosinate)
  • BCH-LMO-SCBD-14750-19 Living Modified Organism MON-ØØ81Ø-6 - YieldGard™ maize
    Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
EN
Characteristics of the modification process
PHI8999A, PV-ZMBK07 and PV-ZMGT10
EN
  • Cross breeding
 
0.610 kb
 
 
0.800 kb
 
 
3.460 kb
 
 
1.980 kb
 
 
1.820 kb
 
 
0.720 kb
 
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
DNA insert from MON810, vectors PV-ZMBK07 and PV-ZMGT10:

MON810 contains a truncated portion of a synthetic form of the cry1Ab gene from Bacillus thuringiensis subsp. Two constructs PV-ZMBK07 and PV-ZMGT10 have been used for transformation, but molecular analyses showed that MON810 does not contain any element from PV-ZMGT10 construct and only the elements from construct PV-ZMBK07 have been integrated into its genome.

DNA insert from TC1507, vector PHI8999A: 

DNA fragment PHI8999A contains two adjacent plant gene expression cassettes for the cry1F and pat genes

For additional information on this LMO, please refer to the records of the parental LMOs.
EN
LMO characteristics
EN
  • Food
  • Feed
Detection method(s)
MON810 contains one integrated DNA consisting of P-e35S, I-Hsp70 and cry1Ab. The terminator of the nopaline synthase (nos) gene was lost due to a truncation at the 3' end of the gene cassette during genome integration and is, therefore, not present in MON810.

Southern analysis using a cry1F probe carried out by the European Food Safety Authority revealed the presence of two cry1F inserts. The first represented the intact gene from the expression cassette. The second insert was a truncated cry1F  fragment of 335bp, which is located at the 5' end of the insertion locus. In addition, analysis of the sequences adjacent to the insert of fragment PHI8999A revealed DNA fragments that correspond to small segments from PHI8999A, including incomplete sequences from the pat gene, the maize ubiquitin promoter and the terminator from Agrobacterium. Furthermore, different fragments of chloroplast DNA and a number of sequences with similarity to retrotransposons are also present in the border region of the insert.
EN
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 17
Risk Assessment generated by a regulatory process Living modified organism(s) 18
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 2