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Date of creation
2012-05-09 13:44 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-05-09 13:45 UTC (dina.abdelhakim@cbd.int)

General Information
 Characterization of cry Genes in a Mexican Bacillus thuringiensis Strain Collection
Alejandra Bravo, Sergio Sarabia, Lorena Lopez, Hernesto Ontiveros, Carolina Abarca, Anabel Ortiz, Miriam Ortiz, Laura Lina, Francisco J. Villalobos, Guadalupe Peña, María-Eugenia Nuñez-Valdez, Mario Soberón, and Rodolfo Quintero
Author’s contact information
Alejandra Bravo
E-mail: bravo@ibt.unam.mx

Departamento de Microbiologı́a Molecular
Instituto de Biotecnologı́a
Universidad Nacional Autónoma de México
Apdo. Postal 510-3, Cuernavaca 62250
Morelos, México

Phone: (52) 73-29 1635
Fax: (52) 73-17 2388
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Summary, abstract or table of contents

Mexico is located in a transition zone between the Nearctic and Neotropical biogeographical regions and contains a rich and unique biodiversity. A total of 496 Bacillus thuringiensis strains were isolated from 503 soil samples collected from the five macroregions of the country. The characterization of the strain collection provided useful information on the ecological patterns of distribution of B. thuringiensis and opportunities for the selection of strains to develop novel bioinsecticidal products. The analysis of the strains was based on multiplex PCR with novel general and specific primers that could detect the cry1,cry3, cry5, cry7, cry8,cry9, cry11, cry12,cry13, cry14, cry21, andcyt genes. The proteins belonging to the Cry1 and Cry9 groups are toxic for lepidopteran insects. The Cry3, Cry7, and Cry8 proteins are active against coleopteran insects. The Cry5, Cry12, Cry13, and Cry14 proteins are nematocidal. The Cry11, Cry21, and Cyt proteins are toxic for dipteran insects. Six pairs of general primers are used in this method. Strains for which unique PCR product profiles were obtained with the general primers were further characterized by additional PCRs with specific primers. Strains containingcry1 genes were the most abundant in our collection (49.5%). Thirty-three different cry1-type profiles were identified. B. thuringiensis strains harboringcry3 genes represented 21.5% of the strains, and 7.9% of the strains contained cry11 and cyt genes.cry7, cry8, and cry9 genes were found in 0.6, 2.4, and 2.6% of the strains, respectively. No strains carrying cry5, cry12, cry13,cry14, or cry21 genes were found. Finally, 14% of the strains did not give any PCR product and did not react with any polyclonal antisera. Our results indicate the presence of strains that may harbor potentially novel Cry proteins as well as strains with combinations of less frequently observed cry genes.
Thematic areas
  • Scientific and technical issues
    • Risk assessment
Background material to the “Guidance on risk assessment of living modified organisms”
Is this document is recommend as background material for the “Guidance on Risk Assessment of Living Modified Organisms”
Section(s) of the “Guidance on Risk Assessment of Living Modified Organisms” this background material is relevant
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
Publisher and its location
American Society for Microbiology
Copyright © 1998, American Society for Microbiology
9 page PDF
Applied and Environmental Microbiology
Keywords and any other relevant information
Citation: Appl. Environ. Microbiol. December 1998 vol. 64 no. 12 4965-4972