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Modified Organism
Potato with altered starch quality
Record information and status
Record ID
103636
Status
Published
Date of creation
2012-06-19 20:37 UTC (manoela.miranda@cbd.int)
Date of last update
2012-06-28 14:49 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-06-28 14:49 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato with altered starch quality
Transformation event
pMFIp9-1, line #1332
Developer(s)
LfL Freising
Institut für Pflanzenbau und Pflanenzüchtung
Bayerische Landesanstalt für Landwirtschaft
Vöttinger Strasse 38

Freising, Bayern
Germany, 85354
Phone:+ 49 8161 71-5800
Fax:+ 49 8161 71-5809
Email:Poststelle@LfL.bayern.de
Url:http://www.lfl.bayern.de/ipz/biotechnologie/33232/index.php
Description
Solanum tuberosum was transformed with a construct containing a 581 bp long fragment of exon 2 of the potato granule bound starch synthase (GBSS) gene in antisense orientation. The transcription of this fragment is controlled by its native, tuber-specific promoter P-gbss and its contiguous non-translated leader sequence - consisting of exon 1 and intron 1 of the GBSS gene. The transcription is terminated by the CaMV 35S terminator from Cauliflower mosaic virus.

The markergen-free amylopectin potato pMFIp9-1, line #1332 exhibits an altered starch quality compared to wild-type potato. Nearly pure amylopectin-starch is synthesized.

By introduction of an antisense-sequence the endogenous GBSS gene is silenced post-transcriptionally. This leads to a significant reduced expression of GBSS-protein and therefore an impaired amylose synthesis; the synthesized starch within these potato lines consists almost exclusively of amylopectin.

This transgenic organism does not contain any marker genes for selection purposes within the inserted T-DNA.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Solanum tuberosum L. of cultivar Walli transformed
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Characteristics of the transformation process
Vector
pMFIp9-1
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Granule bound starch synthase gene promoter
0.00 Kb
 
 
Granule-bound starch synthase gene
581.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
Further details
LMO characteristics
Modified traits
Other gene(s) whose expression was affected by the transformation
Granule-bound starch synthase gene - Solanum tuberosum - Potato, SOLTU
altered carbohydrate composition: increased amylopectin content
How the expression of the gene(s) was affected
The GBSS gene is silenced post-transcriptionally.
Common use(s)
  • Food
  • Feed
  • Research
Detection method(s)
Additional information
PCR-based methods, to be developed on information on the transformation vector.

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record