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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Potato with modified starch composition
EN
Potato (Solanum tuberosum) pHAS3
No
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Person:BASF Plant Science GmbHCarl-Bosch-Str. 38Ludwigshafen,
67056 , GermanyPhone: +49 621 60-0,Fax: +49 621 60-42525,Email: global.info@basf.com,
This genetically modified potato exhibits an altered starch metabolism and tolerance towards herbicides containing the active ingredient imazamox.
The inserted DNA segments from plasmid pHAS3 contain fragments of the coding region of the be1 and be2 gene as a double inverted repeat which is regulated by the gbss promoter and the nos terminator from Agrobacterium tumefaciens. This mediates the expression of a RNAi construct that promotes silencing of the endogeneous be1 and be2-gene. A lack of be1 and be2 activity leads to an increased portion of amylopectin within the potato starch.
As a selection marker system the ahas gene from Arabidopsis thaliana under the control of the nos promoter and nos terminator mediates tolerance towards herbicides containing the active ingredient imazamox.
EN
The inserted DNA segments from plasmid pHAS3 contain fragments of the coding region of the be1 and be2 gene as a double inverted repeat which is regulated by the gbss promoter and the nos terminator from Agrobacterium tumefaciens. This mediates the expression of a RNAi construct that promotes silencing of the endogeneous be1 and be2-gene. A lack of be1 and be2 activity leads to an increased portion of amylopectin within the potato starch.
As a selection marker system the ahas gene from Arabidopsis thaliana under the control of the nos promoter and nos terminator mediates tolerance towards herbicides containing the active ingredient imazamox.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
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BPS-A1Ø2Ø-5 - Amadea potato| BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio), Resistance to herbicides (Imidazolinone, Sulfonylurea), Selectable marker genes and reporter genes
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Potato with altered starch content| BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio), Resistance to herbicides (Sulfonylurea)
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BPS-PHØ48-1 - Fortuna potato| BASF Plant Science GmbH | Resistance to antibiotics (Streptomycin), Resistance to diseases and pests (Fungi), Resistance to herbicides (Imidazolinone, Sulfonylurea)
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Potato modified for resistance to Phytophthora infestans| BASF Plant Science GmbH | Resistance to diseases and pests (Phytophthora infestans resistance), Resistance to herbicides (Sulfonylurea)
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BPS-25271-9 - Amflora™Potato| Reduced amylose in starch content, Resistance to antibiotics (Kanamycin)
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Potato with modified starch composition| BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio), Resistance to antibiotics (Kanamycin), Resistance to herbicides (Imidazolinone, Sulfonylurea)
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Potato modified for altered starch composition| AVEBE | Changes in quality and/or metabolite content (Amylose and amylopectin ratio), Reduced amylose in starch content
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AVE-436G7-1 - Modena potato with altered starch| BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio)
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Potato with altered starch composition| BASF Plant Science GmbH | Changes in quality and/or metabolite content (Amylose and amylopectin ratio)
pHAS3 (derivative of pPZP200)
EN
- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14997-6 Granule bound starch synthase gene promoter | Solanum tuberosum (Potato, SOLTU)Promoter
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BCH-GENE-SCBD-48366-2 Branching Enzyme 1 | Solanum tuberosum (Potato, SOLTU)Protein coding sequence | altered carbohydrate composition: increased amylopectin content
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BCH-GENE-SCBD-48453-2 Branching Enzyme 2 | Solanum tuberosum (Potato, SOLTU)Protein coding sequence | altered carbohydrate composition: increased amylopectin content
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-48073-8 Acetohydroxy acid synthase gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Resistance to herbicides (Imidazolinone, Sulfonylurea)
EN
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BCH-GENE-SCBD-48366-2 Branching Enzyme 1 | Solanum tuberosum (Potato, SOLTU)Protein coding sequence | altered carbohydrate composition: increased amylopectin content
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BCH-GENE-SCBD-48453-2 Branching Enzyme 2 | Solanum tuberosum (Potato, SOLTU)Protein coding sequence | altered carbohydrate composition: increased amylopectin content
A double-stranded RNA is formed in the genetically modified plants; RNA interference causes inactivation of the endogenous transcripts of the respective genes. As a result, formation of the corresponding enzymes is blocked and a starch with reduced amylopectin content is synthesised in the tubers.
EN
- Food
- Feed
- Research
PCR-based methods, to be developed on information on the transformation vector.
EN
EN
- Expression of a chimaeric granule-bound starch synthase-GUS gene in transgenic potato plants [ English ]
- Production of very-high-amylose potato starch by inhibition of SBE A and B [ English ]
- Molecular Basis of Imidazolinone Herbicide Resistance in Arabidopsis thaliana var Columbia.pdf [ English ]
- Molecular Basis of Imidazolinone Herbicide Resistance in Arabidopsis thaliana var Columbia.pdf [ German ]
- Progress in Arabidopsis starch research and potential biotechnological applications [ English ]
- A minor form of starch branching enzyme in potato (So-lanum tuberosum L.) tubers has a major effect on starch structure cloning and charac-terisation of multiple forms of SBE A..pdf [ English ]
- A minor form of starch branching enzyme in potato (So-lanum tuberosum L.) tubers has a major effect on starch structure cloning and charac-terisation of multiple forms of SBE A..pdf [ German ]
- The small, versatile pPZP family of Agrobacte-rium binary vectors for plant transformation. [ English ]
- Herbicide resistant forms of Arabidopsis thaliana acetohydroxyacid synthase characterization of the catalytic properties and sensitivity to inhibitors of four defined mutants..pdf [ English ]
- Herbicide resistant forms of Arabidopsis thaliana acetohydroxyacid synthase characterization of the catalytic properties and sensitivity to inhibitors of four defined mutants..pdf [ German ]
- A novel selection system for potato transformation using a mutated AHAS gene [ English ]
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