Flax modified for insertional mutagenesis | BCH-LMO-SCBD-104315 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 24 Oct 2012
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Flax modified for insertional mutagenesis
Flax and linseed plants were genetically modified to induce mutations of existing plant genes using a vector for insertion mutagenesis. The vector contained a 35S tetramer enhancer fragment which, upon insertion, can cause an activation of the genes adjacent to the T-DNA insertion place, i.e. a dominant mutation. On the other hand the T-DNA integration into an active gene area may cause the inactivation of this gene. This change is then expressed as a recessive mutation. Theoretically these random mutations may result in changes in the plant's metabolism and in specific cases of it's morphological characteristics or the biological properties which may be useful for crop improvement.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
Binary vector GV3101 (pPM90RK)(pPCVRN4)
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The DNA segment inserted by the binary vector GV3101 (pPM90RK)(pPCVRN4) – does not contain any gene with direct effect on the recipient properties, other than a selectable marker, hpt, that confers resistance to hygromycin for the identification of transformed genotypes.
LMO characteristics
  • Research
Detection method(s)
Additional Information
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Risk Assessment generated by a regulatory process Living modified organism(s) 1