Flax modified for altered fatty acid composition | BCH-LMO-SCBD-104324 | Living Modified Organism | Biosafety Clearing-House
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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 24 Oct 2012
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Flax modified for altered fatty acid composition
EN
Transformation of flax aimed at fatty acid desaturase 2 (FAD2) gene inactivation by RNA interference
No
Flax plants were transformed with a vector construct bearing expression cassette with two FAD2 gene (coding for type 2 fatty acids desaturase) segments modified in a way to employ RNA interference system for suppression of desaturase function, and due to it to increase the content of oleic acid in linseed oil.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pPCV812i/35S/FAD2i/35S/GUS (FAD2-i GUS)
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
  • BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)
    Protein coding sequence | Selectable marker genes and reporter genes
  • BCH-GENE-SCBD-104323-2 Fatty Acid Desaturase 2 | Linum usitatissimum (Flax, Flax, Linseed, LINUS)
    Protein coding sequence | Changes in quality and/or metabolite content (Lipid and fatty acids)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-14975-5 Beta-lactamase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Ampicillin)
The inserted plasmid construct contains the FAD2-i expression cassette (the CaMV 35S promoter, DNA segment for desaturase, spacer, inverted DNA segment), CaMV 35S promoter from pAN4 plasmid, hpt gene from E. Coli, uidA gene for beta-glucuronidase (GUS) together with E.coli intron (K12) and AmpR gene for plasmid selection in bacteria.

Along with modified FAD2 gene the insert contains hpt selectable gene to enable selection of transgenic plants, uidA (GUS) reporter gene and AmpR gene to support plasmid construct selection in bacteria.
EN
LMO characteristics
EN
  • Research
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1

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