IFD-25947-1 - Moonpearl carnation | BCH-LMO-SCBD-104608 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 14 Jan 2013 last updated: 28 Aug 2014
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Moonpearl carnation
EN
25947
Yes
IFD-25947-1
Moonpearl carnation has flowers with a unique violet/mauve colour due to the biosynthesis of the anthocyanin pigment delphinidin. The transgenic lines were derived from the parent cultivar which is a pink coloured carnation.

The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) from petunia and a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H). Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations.

A Dihydroflavonol-4-reductase sequence from carnation has been utilised in a dsDFR format in order to suppress expression of the endogenous carnation DFR gene, thus allowing dominant expression of the introduced petunia Dihydroflavonol-4-reductase.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
  • IFD-25958-3 - Moonberry carnation
    | Dr Yoshikazu Tanaka | Changes in quality and/or metabolite content (Flavonoids (e.g. anthocyanin), Pigmentation / Coloration), Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
Characteristics of the modification process
pCGP3366
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The dihydroflavonol-4-reductase sequence from carnation is inserted in a sense/ antisense orientation. The transcription product results in the formation of a dsDFR which suppress the expression of the endogenous DFR gene, thus allowing dominant expression of the introduced petunia Dihydroflavonol-4-reductase thus leading to the synthesis of delphinidin imparting a violet/mauve colour to the carnation.

The T-DNA is present at one integration locus and contains one copy of each T-DNA component as determined by Southern blot analysis
EN
LMO characteristics
EN
  • Ornamental
Detection method(s)
EN
Additional Information
Moonpearl was developed using recombinant DNA techniques to produce flowers with a unique violet/mauve colour by introducing three genes that function together in the biosynthesis of the anthocyanin pigment delphinidin.

The transgenic lines were derived from the parent cultivar which is a pink coloured carnation.The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) and a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H), a member of the NADPH-Cytochrome P450 reductase family.

Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations. Expression of a dsDFR construct suppresses the activity of endogenous DFR.
EN
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Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 3
Country's Decision or any other Communication Living modified organism(s) 3
Living Modified Organism Related LMO(s) 2