Record details

Living Modified Organism identity

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LMO name

Agrisure® 3122

Transformation event

BT11 x DAS-59122 x MIR604 x TC1507 x GA21

Unique identifier

SYN-BTØ11-1 x DAS-59122-7 x SYN-IR6Ø4-5 x DAS-Ø15Ø7-1 x MON-ØØØ21-9

Developer(s)

Record #38695

Syngenta Japan K.K.

401-2, Mukounodai, Takatsuhara, Tako-machi,

Katori-gun, Chiba

Japan, 289-2304

Url:	http://www.syngentaseeds.co.jp/

Description

A stacked insect-resistant and herbicide-tolerant maize derived from conventional cross-breeding of BT11 x DAS-59122 x MIR604 x TC1507 x GA21. Insect resistance is conferred by the cry1A(b), cryIF, cry34Ab1, cry3A, and cry35Ab1 genes from the common soil bacterium Bacillus thuringiensis, and glufosinate and glyphosate herbicide tolerance through the pat and EPSPS genes respectively.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #246

Zea mays - Maize, Corn, MAIZE

Record #14794

MON-ØØØ21-9 - Roundup Ready™ maize

Resistance to herbicides - Glyphosate

Show detection method(s)

Record #15105

SYN-IR6Ø4-5 - Agrisure™ RW Rootworm-Protected maize

Mannose tolerance Resistance to diseases and pests - Insects - Coleoptera (beetles) - Western corn rootworm (Diabrotica virgifera) Selectable marker genes and reporter genes

Show detection method(s)

Record #14797

SYN-BTØ11-1 - YieldGard™ maize

Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate

Show detection method(s)

Record #15165

DAS-59122-7 - Herculex™ RW Rootworm Protection maize

Resistance to diseases and pests - Insects - Coleoptera (beetles) Resistance to herbicides - Glufosinate

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Record #14841

DAS-Ø15Ø7-1 - Herculex™ I maize

Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate

Show detection method(s)

Characteristics of the transformation process

Vector

pZO1502, pDPG434, pZM26, PHP17662 and PHI8999A

Techniques used for the modification

Cross breeding

Genetic elements construct

Ubiquitin gene promoter

#100362

1.98 Kb

Cry1F

#14987

1.82 Kb

ORF25 PolyA Terminator sequence

#100363

0.72 Kb

CaMV 35S promoter

#100287

0.55 Kb

Phosphinothricin N-acetyltransferase gene

#15002

0.55 Kb

CaMV 35S terminator

#100290

0.20 Kb

CaMV 35S promoter

#100287

0.51 Kb

Alcohol Dehydrogenase 1, intron 6

#103625

0.47 Kb

Cry1Ab

#14985

1.85 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

CaMV 35S promoter

#100287

0.42 Kb

Alcohol Dehydrogenase 1, intron 2

#103867

0.18 Kb

Phosphinothricin N-acetyltransferase gene

#15002

0.55 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

Rice actin 1 gene promoter

#100364

1.37 Kb

Rice actin 1, intron

#100355

0.00 Kb

Optimized Transit Peptide

#101419

0.37 Kb

5-enolpyruvylshikimate-3-phosphate synthase

#46333

1.34 Kb

Nopaline Synthase Gene Terminator

#100269

0.24 Kb

Metallothionein-like gene promoter

#103881

0.00 Kb

mCry3A

#43634

0.00 Kb

Nopaline Synthase Gene Terminator

#100269

0.00 Kb

Ubiquitin gene promoter

#100362

0.00 Kb

Ubiquitin Intron 1

#103627

0.00 Kb

Phosphomannose Isomerase gene

#15003

0.00 Kb

Nopaline Synthase Gene Terminator

#100269

0.00 Kb

Ubiquitin gene promoter

#100362

0.00 Kb

Cry34Ab1

#14994

0.00 Kb

Proteinase inhibitor II gene terminator

#100367

0.00 Kb

Peroxidase gene promoter

#100368

0.00 Kb

Cry35Ab1

#14995

0.00 Kb

Proteinase inhibitor II gene terminator

#100367

0.00 Kb

CaMV 35S promoter

#100287

0.00 Kb

Phosphinothricin N-acetyltransferase gene

#15002

0.00 Kb

CaMV 35S terminator

#100290

0.00 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

DNA insert from Bt 11 vector pZO1502
Contributed the cry1Ab gene to confer resistance to the European corn borer (Ostrinia nubilalis), and the phosphinothricin N-acetyltransferase (PAT) encoding gene to confer tolerance to phosphinothricin (PPT) herbicide, specifically glufosinate ammonium.

DNA insert from GA21 vector pDPG434
The 5-enolpyruvyl shikimate-3-phosphate synthase (epsps) gene from maize was modified through site-directed mutagenesis, such that its encoded enzyme was insensitive to inactivation by glyphosate. The resulting gene was used to develop GA21

DNA insert from MIR604 vector pZM26
Cry3A gene was modified for enhanced expression in maize and such that the amino acid sequence of the synthetic version of Cry3A is the same as the native protein, except for the modified serine-protease recognition site. The pmi gene encodes the enzyme phosphomannose isomerase (PMI) that allows the plants to utilise mannose as a carbon source and is used as a selectable marker.

DNA insert from 59122 vector PHP17662
The cry34Ab1 and cry35Ab1 genes, isolated from the common soil bacterium Bacillus thuringiensis (Bt) strain PS149B1, produce the insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1. The pat gene was isolated from the soil bacterium Streptomyces viridochromogenes and confers tolerance to herbicides containing glufosinate ammonium.

DNA insert from TC1507 vector PHI8999A
TC1507 modified with the insertion of the Cry1F geneto confer resistance to the European corn borer (Ostrinia nubilalis). A transformation cassette coding for phosphinothricin (PPT) herbicide tolerance, specifically glufosinate ammonium, was also inserted into the organism.

For additional information on this LMO, please refer to the records of the parental LMOs.

LMO characteristics

Modified traits

Resistance to diseases and pests
- Insects
  - Coleoptera (beetles)
    - Western corn rootworm (Diabrotica virgifera)
    - Northern corn rootworm (Diabrotica barberi)
  - Lepidoptera (butterflies and moths)
Resistance to herbicides
- Glufosinate
- Glyphosate

Common use(s)

Food
Feed

Detection method(s)

External link(s)

SYN-BTØ11-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

MON-ØØØ21-9 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

SYN-IR6Ø4-5 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

DAS-59122-7 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

DAS-Ø15Ø7-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

Additional Information

Other relevant website address or attached documents

BT11 x DAS-59122 x MIR604 x TC1507 x GA21 (Japanese)