DAS-68416-4 - Enlist™ Soybeans | BCH-LMO-SCBD-104808 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 27 Mar 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Enlist™ Soybeans
DAS-68416-4 soybean was modified with the insertion of a gene encoding aryloxyalkanoate dioxygenase and phosphinothricin N-acetyltransferase to confer tolerance to the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and glufosinate respectively.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Aryloxyalkanoate dioxygenase gene sequence from Delftia acidovorans was plant-optimized. The native and plant-optimized DNA sequences of aad-12 are 79.7% identical.

Southern blot analysis indicated that a single, intact insert of the aad-12 and pat genes were stably integrated into the soybean genome. Southern analyses also indicated the absence of the plasmid backbone DNA in DAS-68416-4 soybean.
LMO characteristics
  • Food
  • Feed
Detection method(s)
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 13
Country's Decision or any other Communication Living modified organism(s) 10
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 4
Living Modified Organism Recipient Organism” or “Parental Organisms 1