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Modified Organism
DAS-68416-4 - Enlist™ Soybeans
Record information and status
Record ID
104808
Status
Published
Date of creation
2013-03-27 15:58 UTC (dina.abdelhakim@cbd.int)
Date of last update
2013-03-27 16:14 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-03-27 16:14 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Enlist™ Soybeans
Transformation event
DAS-68416-4
Unique identifier
DAS-68416-4
Developer(s)
Dow AgroSciences GmbH
Dow AgroSciences GmbH
Truderinger Straße 15
München, Bayern
Germany, 81677
Phone:+ 49 89 - 4 55 33 - 0
Fax:+ 49 89 - 4 55 33 - 111
Email:DowAgroSciencesD@dow.com
Url:http://www.dowagro.com/de
Description
DAS-68416-4 soybean was modified with the insertion of a gene encoding aryloxyalkanoate dioxygenase and phosphinothricin N-acetyltransferase to confer tolerance to the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and glufosinate respectively.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
Characteristics of the transformation process
Vector
pDAB4468
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
RB7 matrix attachment region
1.17 Kb
 
 
Polyubiquitin10 gene promoter
1.32 Kb
 
 
Aryloxyalkanoate dioxygenase gene
0.88 Kb
 
 
ORF23 3' Untranslated region
0.46 Kb
 
 
CsVMV promoter
0.52 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
ORF1 3' Untranslated region
0.70 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Aryloxyalkanoate dioxygenase gene sequence from Delftia acidovorans was plant-optimized. The native and plant-optimized DNA sequences of aad-12 are 79.7% identical.

Southern blot analysis indicated that a single, intact insert of the aad-12 and pat genes were stably integrated into the soybean genome. Southern analyses also indicated the absence of the plasmid backbone DNA in DAS-68416-4 soybean.
LMO characteristics
Modified traits
  • Tolerance to 2,4-Dichlorophenoxyacetic acid
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (20)
IDDescription
20record(s) found
Country's Decision or any other Communication9 records
Modified Organism1 record
Organization1 record
Risk Assessment9 records

   
   
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