Record details

Living Modified Organism identity

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LMO name

Enlist™ Maize

Transformation event

DAS-40278-9

Unique identifier

DAS-4Ø278-9

Developer(s)

Record #104809

Dow AgroSciences GmbH

Truderinger Straße 15

München, Bayern

Germany, 81677

Phone:	+ 49 89 - 4 55 33 - 0
Fax:	+ 49 89 - 4 55 33 - 111
Email:	DowAgroSciencesD@dow.com
Url:	http://www.dowagro.com/de

Description

DAS-40278-9 corn was modified with the insertion of a plant optimised coding sequence for aryloxyalkanoate dioxygenase to confer tolerance to the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and aryloxyphenoxypropionate (AOPP) acetyl coenzyme A carboxylase (ACCase) inhibitors.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #246

Zea mays - Maize, Corn, MAIZE

Characteristics of the transformation process

Vector

pDAS1740

Techniques used for the modification

Direct DNA transfer

Genetic elements construct

RB7 matrix attachment region

#104795

1.17 Kb

Ubiquitin gene promoter

#100362

1.99 Kb

Aryloxyalkanoate dioxygenase gene

#104812

0.89 Kb

Per5 3' Untranslated Region

#104813

0.37 Kb

RB7 matrix attachment region

#104795

1.17 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

The LMO was generated using the Whiskers mediated transformation method. The aad-1 coding sequence was modified for plant-optimized expression.

Southern blot analysis indicated that a single complete copy of the transformation cassette was stably integrated into the host genome at a single locus. Additionally tests showed that there was no integration of segments from the vector backbone.

LMO characteristics

Modified traits

Resistance to herbicides
Tolerance to 2,4-Dichlorophenoxyacetic acid
Tolerance to aryloxyphenoxypropionate

Common use(s)

Food
Feed

Detection method(s)

External link(s)

DAS-40278-9 - EU Database of Reference Methods for GMO Analysis

Additional Information

Other relevant website address or attached documents

DAS-4Ø278-9 - OECD

DAS-40278-9 - Dow.pdf