DP-Ø61Ø61-7 - Glyphosate tolerant canola | BCH-LMO-SCBD-105040 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 18 Sep 2013 last updated: 18 Feb 2021
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Glyphosate tolerant canola
  • - Organization: Pioneer Hi-Bred International Inc. () | BCH-CON-SCBD-14931-2
    Pioneer Hi-Bred International Inc. ()
    Private sector (business and industry)
    7100 NW 62nd Avenue PO Box 1000
    Johnston, Iowa
    50131, United States of America
    Phone: +1 515 535-3200,
    Website: www.pioneer.com/,
The canola (Brassica rapa) was modified for tolerance to the glyphosate herbicide through the insertion of the Bacillus licheniformis glyphosate-N-acteyltransferase gene, which inactivates glyphosate through acetylation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
  • Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The canola contains one gene cassette for Bacillus licheniformis glyphosate-N-ccteyltransferase (gat). The coding sequence was under control of an Arabidopsis thaliana ubiquitin-10 promoter and a Solanum tuberonsum proteinase inhibitor II 3' untranslated region.

The gat coding sequence expresses the GAT4621 version of the protein, which was functionally improved by a gene shuffling process to optimize the kinetics of glyphosate acetyltransferase.

Southern blot analysis indicated that a single, intact PHP28181 DNA fragment was inserted into the genome with no plasmid backbone DNA.
LMO characteristics
  • Food
  • Feed
Detection method(s)
See patent in additional information section below
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 2
Country's Decision or any other Communication Living modified organism(s) 1
Living Modified Organism Related LMO(s) 1