DAS-444Ø6-6 - Enlist E3™ Soybean | BCH-LMO-SCBD-105041 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 18 Sep 2013 last updated: 17 Aug 2017
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Enlist E3™ Soybean
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DAS-44406-6
Yes
DAS-444Ø6-6
DAS-44406-6 soybean was modified with the insertion of a gene encoding aryloxyalkanoate dioxygenase and phosphinothricin N-acetyltransferase to confer tolerance to the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and glufosinate respectively. Further more a gene encoding a modified version of the EPSPS gene was inserted to confer tolerance to glyphosate.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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Characteristics of the modification process
pDAB8264
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  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-104802-5 Polyubiquitin10 gene promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-104805-2 Aryloxyalkanoate dioxygenase gene | Delftia acidovorans (DELAC)
    Protein coding sequence | Resistance to herbicides
  • BCH-GENE-SCBD-104806-3 ORF23 3' Untranslated region | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-101900-6 CsVMV promoter | Cassava vein mosaic virus (Cassava vein mosaic virus, CVMV, CsVMV)
    Promoter
  • BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-104807-2 ORF1 3' Untranslated region | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-104795-4 RB7 matrix attachment region | Nicotiana tabacum (Tobacco, TOBAC )
    Enhancer
  • BCH-GENE-SCBD-104646-4 Histone H4 gene 3' UTR | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Terminator
  • BCH-GENE-SCBD-46333-8 5-enolpyruvylshikimate-3-phosphate synthase | Zea mays (Maize, Corn, MAIZE)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-101419-4 Optimized Transit Peptide
    Transit signal
  • BCH-GENE-SCBD-104647-3 Histone H4 gene Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-104648-2 Histone H3 Gene II intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Intron
Molecular characterization by Southern blot analyses of DAS-444Ø6-6 soybean confirmed that a single, intact DNA insert containing the aad-12, 2mepsps and pat gene expression cassettes was stably integrated into the soybean genome. Southern blot analyses also confirmed the absence of the plasmid backbone DNA in DAS-444Ø6-6 soybean. The integrity of the inserted DNA was demonstrated in five different breeding generations. Data from segregating generations confirmed the predicted Mendelian inheritance pattern. These data confirmed the stability of DAS-444Ø6-6 soybean during traditional breeding procedures.

The H4A748 promoter also includes an intron from the Histone 3 gene from Arabidopsis thaliana.
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LMO characteristics
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  • Food
  • Feed
Detection method(s)
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Additional Information
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