The Cry1Ac and Cry1F sequences are synthetic versions of the native genes.

The cry1Fv3 gene is comprised of three parts; at the 5′ end, a toxin core that was optimized from the native cry1Fa2 gene originally isolated from Bacillus thuringiensis subsp. aizawai strain PS811; in the middle, a very small portion of cry1Ca3 which was originally isolated from B. thuringiensis subsp. aizawai strain PS811; and at the 3′ end, a tail that was optimized from the native cry1Ab1 tail originally isolated from B. thuringiensis subsp. Berliner 1715. The cry1Fv3 gene encodes the Cry1F protein that is comprised of 1148 amino acids and has a molecular weight of ~130.2 kDa. The amino acid sequence of the Cry1F protein is identical to that expressed in cotton event DAS-24236-5.

The cry1Ac(synpro) gene is comprised of three parts; at the 5′ end, a toxin core that was optimized from the native cry1Ac1 gene originally isolated from B. thuringiensis subsp. kurstakistrain HD73; in the middle, a very small portion of cry1Ca3 which was originally isolated from B. thuringiensis subsp. aizawai strain PS811; and at the 3′ end, a tail that was optimized from the native cry1Ab1 tail originally isolated from B. thuringiensis subsp. Berliner 1715. Thecry1Ac(synpro) gene encodes the Cry1Ac protein that is comprised of 1156 amino acids and has a molecular weight of ~130.7 kDa. The cry1Ac(synpro) gene sequence and the corresponding Cry1Ac amino acid sequence are identical to that expressed in cotton event DAS-21023-5

Molecular characterization by Southern blot analyses of DAS-81419-2 soybean confirmed that a single, intact DNA insert containing the cry1Ac, cry1F, and pat gene expression cassettes was integrated into the soybean genome and the intact DNA insert was stably inherited in the five breeding generations tested. Southern blot analyses confirmed the absence of the plasmid backbone DNA in DAS-81419-2 soybean.